Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
권호 표지

The Safety and Clinical Test of Anti-Helicobacter pylori IgY

Anti-Helicobacter pylori IgY의 안전성 및 임상적 효과

  • Published : 2005.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to evaluate the effectiveness of anti-Helicobacter pylori IgY powder to suppress infection of Helicobacter pylori in humans. Hens were immunized with H. pylori to produce a specific anti-H. pylori IgY in their egg yolks, and then anti-H. pylori IgY Powder was used a sample after egg yolks were harvested The safety tests of anti-H. pylori IgY powder were conducted a acute and subacute toxicity test, The result was that the mice fed IgY powder were normal state on a acute and subacute toxicity test The effect of anti-H. pylori IgY powder was evaluated by urease breath test, Volunteers who tested positive for H. pylori using a $^{13}C-urea$ breath test were divided in two groups, one was administrated with anti-H. pylori IgY powder (11.2g/day) and natural extract mixture and the other was administrated with water soluble protein fraction (3.2g/day) of anti-H. pylori IgY powder, The results of clinical test in two groups were shown reduction of UBT value about 23 and 18 respectively. This result indicates that anit-H. pylori IgY is safety and can be used toy the effective supplement as an ingredient of functional food.

본 연구는 H. pylori를 산란계에 과면역시켜서 얻은 anti-H pylori IgY난황 분말의 안전성과 위염 환자에 있어서 H. pylori에 대한 억제 효과를 알아보기 위하여 실시하였다. Anti-H. pylori IgY난황 분말의 안전성 실험은 급성 독성실험과 아급성 독성실험으로 알아보았다. 급성 독성 실험시 식이섭취나 성장 모두 정상적이었고, 마우스의 체중(kg)당 400mg까지 투여했을 때 이상을 관찰할 수 없었다. 아급성 독성실험에서 외관상의 이상현상은 관찰할 수 없었고, 혈청의 CBC 검사에서 백혈구 수치도 정상적이었다. 그리고 소화기관과 간조직의 병리학적 이상은 관찰되지 않았다. 이 결과로 미루어 anti-H pylori IgY난황분말은 독성이 없음이 확인되었다. 임상실험에서는 WSF 분말 급이군의 UBT 수치는 58.65에서 35.33으로 약 23.31 감소하였으며, anti-H pylori IgY 난황분말 혼합 급이군의 UBT 수치는 47.77에서 28.81로 약 17.95 감소 효과를 보였다. WSF 분말 급이군은 anti-H. pylori IgY 난황분말 혼합 급이군보다 UBT 수치의 감소율이 더 크게 나타났다. 이와 같이 임상실험에서 anti-H, pylori IgY는 H. pylori의 억제 효과가 있음이 확인되었다.

Keywords

References

  1. Brenner, H., Rothenbacher, D., Bode, G., and Adler, G. (1999) Inverse graded relation between alcohol consumption and active infection with Helicobacter pylori. Am. J. Epidemiol. 149: 571-576 https://doi.org/10.1093/oxfordjournals.aje.a009854
  2. Correa, P., Fontham, E. T., Bravo, J. C., Bravo, L. E., Ruiz, B., Zarama, G., Realpe, J. L., Malcom, G. T., Li, D., Johnson, W. D., and Mera, R. (2000) Chemoprevention of gastric dysplasia: randomized trial of antioxidant supplements and anti-Helicobacter pylori therapy. J. Natl. Cancer Inst. 92: 1881-1888 https://doi.org/10.1093/jnci/92.23.1881
  3. Fassina, G., Verdoliva, A., Palombo, G., Ruvo, M., and Cassani, G. (1998) Immunoglobulin specificity of TG19318: a novel synthetic ligand for antibody affinity purification. J. Mol Recognit. 11: 128-133 https://doi.org/10.1002/(SICI)1099-1352(199812)11:1/6<128::AID-JMR408>3.0.CO;2-8
  4. Hopkins, R. J., Girardi, L. S., and Turney, E. A. (1996) Relationship between Helicobacter pylori eradication and reduced duodenal and gastric ulcer recurrence. Gastroenterology 110: 1244-1252 https://doi.org/10.1053/gast.1996.v110.pm8613015
  5. Horie, K., Horie, N., Abdou, A. M., Yang, J. O., Yun, S. S., Chun, H. N., Park, C. K., Kim, M., and Hatta, H. (2004) Suppressive effect of functional drinking yogurt containing specific egg yolk immunoglobulin on Helicobacter pylori in humans. J. Dairy Sci. 87: 4073-4079 https://doi.org/10.3168/jds.S0022-0302(04)73549-3
  6. Hunt, R. H. (1996) Eradication of Helicobacter pylori infection. Am. J. Med. 100(5A): 42S-50S
  7. Ma, J. K., Hunjan, M., Smith, R., and Lehner, T. (1989) Specificity of monoclonal antibodies in local passive immunization against Streptococcus mutans. Clin. Exp. Immunol. 77: 331-337
  8. Mabe, K., Yamada, M., Oguni, I., and Takahashi, T. (1999) In vitro and in vivo activities of tea catechins against Helicobacter pylori. Antimicrob. Agents Chemother. 43: 1788-91
  9. NIH (National Institute of Health) Consensus Conference. (1994) Helicobacter pylori in peptic ulcer disease. JAMA 272: 65-69 https://doi.org/10.1001/jama.272.1.65
  10. Otake, S., Nishihara, Y., Makimura, M., Hatta, H., Kim, M., Yamamoto, T., and Hirasawa, M. (1991). Protection of rats against dental caries by passive immunization with hen-egg-yolk antibody (IgY). J. Dent. Res. 70: 162-166 https://doi.org/10.1177/00220345910700030101
  11. Owen, R. J. (1998) Helicobacter-species classification and identification. Br. Med. Bull. 54: 17-30 https://doi.org/10.1093/oxfordjournals.bmb.a011667
  12. Park, M. J., Kim, J. S. and Yim, J. Y (2001) The effect of a fermented milk containing Lactobacilli on Helicobacter pylori in human gastric mocosa. Korean J. Gastroenterol. 38: 233-240
  13. Parsonnet, J., Friedman, G. D., Vandersteen, D. P., Chang, Y., Vogelman, J. H., Orentreich, N., and Sibley, R. K. (1991) Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325: 1127-1131 https://doi.org/10.1056/NEJM199110173251603
  14. Parsonnet, J., Hansen, S., Rodriguez, L., Gelb, A. B., Warnke, R. A., Jellum, E., Orentreich, N., Vogelman, J. H., and Friedman, G. D. (1994) Helicobacter pylori infection and gastric lymphoma. N. Engl. J. Med. 330: 1267-1271 https://doi.org/10.1056/NEJM199405053301803
  15. Patterson, R., Youngner, J. S., Weigle, W. O., and Dixon, F. J. (1962) Antibody production and transfer to egg yolk in chickens. J. Immunol. 89: 272-278
  16. Peterson, W. L. (1991) Helicobacter pylori and peptic ulcer disease. N. Engl. J. Med. 324: 1043-1048 https://doi.org/10.1056/NEJM199104113241507
  17. Rhee, K. H. and Yoon, H. S. (1990) Prevalence of Helicobacter pylori infection in Korea. J. Korean Soc. Microbiol. 25: 475-490
  18. Suzuki, H., Nomura, S., Masaoka, T., Goshima, H., Kamata, N., Kodama, Y., Ishii, H., Kitajima, M., Nomoto, K., and Hibi, T. (2004) Effect of dietary anti-Helicobacter pylori-urease immunoglobulin Y on Helicobacter pylori infection. Aliment. Pharmacol. Ther. 1: 185-192
  19. Warren, J. R. and Marshall, B. (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1: 1273-1275