Reproductive and Developmental Biology

  • 제29권4호
  • /
  • Pages.269-271
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  • 2005
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Diagnosis of Freemartinism in Korean Native Cattle by Amplification of Two Different Male-Specific DNA Sequences

  • Kim So Sub (Department of Physiology, Dankook University School of Medicine) ;
  • Yoon Ji Young (Department of Physiology, Dankook University School of Medicine) ;
  • Ahn Kwang Sung (Department of Physiology, Dankook University School of Medicine) ;
  • Shim Hosup (Department of Physiology, Dankook University School of Medicine)
  • 발행 : 2005.12.01
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초록

The freemartinism is the most frequent form of intersexuality found in cattle, and females of heterosexual twins become sterile. With increase of twinning rates due to transfer of multiple embryos derived from in vitro fertilization, it is of great economic value to establish early diagnosis of freemartins to remove infertile individuals from breeding stock. In the present study polymerase chain reaction (PCR) of two different Y-chromosome specific segments (BRY.l and AMX/Y) was performed to identify freemartins from twins and less common single born freemartins in Korean Native Cattle (KNC). Two male-specific sequences were amplified in all heterosexual twins tested (n=5). In addition, Y-specific PCR products were detectable in one of the single born females (n=4) with visible genital abnormalities. These results suggest that the sensitivity of PCR-based assay may be sufficient to detect freemartinism in single born females as well as female partners of heterosexual twins in KNC.

키워드

참고문헌

  1. Ennis S, Gallagher (1994): A PCR-based sex-determination assay in cattle based on the bovine amelogenin locus. Anim Genet 25:425-427 https://doi.org/10.1111/j.1365-2052.1994.tb00533.x
  2. Ennis S, Vaughan L, Gallagher TF (1999): The diagnosis of freemartinism in cattle using sex-specific DNA sequences. Res Vet Sci 67:111-112 https://doi.org/10.1053/rvsc.1998.0286
  3. Laster DB, Turman EJ, Johnson BH, Stephens DF, Renbarger RD (1971): Effect of sex ratio in utero on degree of transformation and chimerism in neonatal bovine freemartins. J Reprod Fertil 24:361-367 https://doi.org/10.1530/jrf.0.0240361
  4. Marcum JB (1974): The freemartin syndrome. Animal Breeding Abstract 42:227-241
  5. Padula AM (2005): The freemartin syndrome: an update. Anim Reprod Sci 87:93-109 https://doi.org/10.1016/j.anireprosci.2004.09.008
  6. Parkinson TJ, Smith KC, Long SE, Douthwaite JA, Mann GE, Knight PG (2001): Inter-relationships among gonadotrophins, reproductive steroids and inhibin in freemartin ewes. Reproduction 122:397-409 https://doi.org/10.1530/rep.0.1220397
  7. Reick GW (1963): Early recognition of reproductive capacity in females of mixed twins in cattle. Praktische Tierarzt 40:89-92
  8. Stewart-Scott IA, Pearce PD, Moore GH, Fennessy PF (1990): Freemartinism in red deer (Cervus elaphus L.). Cytogenet Cell Genet 54:58-59 https://doi.org/10.1159/000132955