International Journal of Industrial Entomology and Biomaterials

  • 제11권2호
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  • Pages.125-127
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  • 2005
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

Construction of a High-efficiency Shuttle Vector Containing the Minimal Replication Origin of Bacillus thuringiensis

  • Kang Joong Nam (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kim Yang-Su (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Wang Yong (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi Heekyu (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Li Ming Shun (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Shin Sang Chul (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Jin Byung Rae (College of Natural Resource and Life Science, Dong-A University) ;
  • Roh Jong Yul (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi Jae Young (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Je Yeon Ho (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
  • 발행 : 2005.12.01
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초록

In order to improve the transformation efficiency of the Bacillus thuringiensis (Bt)-Escherichia coli (E. coli) shuttle vector, pHT3101, we intended to minimize replication origin of Bt in pHT3101. For this, two modified shuttle vectors, pHT1K and pHT261, in which 2.9 kb of replication origin of Bt were shortened to 1 kb and 261 bp, respectively as previously reported. Whereas the pHT1K could efficiently transform Bt into the antibiotic resistant, no transformants were obtained with pHT261. Furthermore, pHT1K showed higher transformation efficiency compared to that of parent vector, pHT3101. Therefore, pHT1K might be a very useful Bt-E. coli shuttle vector carrying minimal replication origin of Bt.

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참고문헌

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