Prediction of Cryptosporidium parvum Inactivation in Advanced Ozone Drinking Water Treatment with Lab Scale Experiments

실험실 규모 크립토스포리디움의 불활성화 실험을 통한 오존 고도정수처리 정수장에서 소독 효과 예측

  • Received : 2004.07.26
  • Accepted : 2004.11.29
  • Published : 2005.01.30

Abstract

With the appearance of pathogenic microorganisms, which were resistant to free chlorine, the significant attention to the necessity of powerful alternative disinfection methods such as ozone, chlorine dioxide, LTV irradiation to inactivating pathogens has been increased in water treatment. Among these alternative disinfection methods, ozone is well known as strong biocidal method and the usage of ozone is also increasing in Korea. However, in Korea, there has been no report on the quantitative study of Cryptosporidium parvum with ozone and its evaluation in advanced drinking water treatments. This study reports on the methodology for predicting the ozone inactivation of Cryptosporidium parvum by ozone disinfection in advanced drinking water treatment. The method is based on the fact that a specific inactivation level of microorganisms is achieved at a unique value of ozone exposures, independent of ozone dose and type of water, and quantitatively described by a delayed Chick-Watson model. The required values ${\bar{C}}T$ for 2 log inactivation of Cryptosporidium parvum was $6.0mg/L{\cdot}min$ and $15.5mg/L{\cdot}min$ at $20^{\circ}C$ and $5^{\circ}C$, respectively. From this obtained Cryptosporidium parvum inactivation curves and calculated ${\bar{C}}T$ values of advanced drinking water treatment water in Korea with FIA (Flow injection alaysis), we can predict that water treatment plant can achieve a 1.1~1.8 log inactivation and 0~0.4 log inactivation at $20^{\circ}C$ and $5^{\circ}C$, respectively. This methodology will be useful for drinking water treatment plants which intend to evaluate the disinfection efficiencies of their ozonation process without full scale test and direct experiments with Cryptosporidium parvum.

Keywords

References

  1. 국립환경연구원, 정수장의 원생동물 시스트 제어연구 (I), (2002)
  2. 국립환경연구원, 정수장의 원생동물 시스트 제어연구 (II), (2003)
  3. 김이호, 크립토스포리디움의 배양 및 정제기술, 첨단환경기술, 8월호, pp. 6-14 (2003)
  4. 박훈수, 염철민, 윤제용, 국내 정수처리공정에서 오존 공정 시설의 현황과 특성, 상하수도학회지, 15(4), pp. 279-292 (2001 )
  5. 윤제용, 손진식, 최숭일, 고도정수처리 정책방향에 관한 연 구, 환경부 (2004)
  6. 이목영, 조은주, 변승헌, 김태호, 오세종, 안숭구, 응집.침 전공정에서의 크립토스포리디움 및 지아디아 제거율 평 가. 대한환경공학회지, 26(4), pp. 397-403 (2004)
  7. 조민, 김지연, 윤제용, UV 조사에 의한 미생물의 불활성화, 첨단환경기술, 2 월호, pp. 5-18 (2004)
  8. 최승일, 윤제용, 정수처리 기준의 의미와 준비현황, 상하수 도 협회지, 봄, pp, 28-33 (2004)
  9. Camel, V. and Bermond, A., The use of ozone and associated oxidation processes in drinking water treatment, Water Res., 32, pp. 3208-3222 (1998). https://doi.org/10.1016/S0043-1354(98)00130-4
  10. Cho, M., Chung, H., and Yoon, J., Disinfection of water containing natural organic matter by using ozone-initiated radical reactions, Appl. Environ. Microbiol., 69, pp. 2284-2291 (2003a) https://doi.org/10.1128/AEM.69.4.2284-2291.2003
  11. Cho, M., Chung, H., and Yoon, J., Quantitative evaluation of the synergistic sequential inactivation of Bacillus subtilis spores with ozone followed by chlorine, Environ. Sci. Tech., 37, pp. 2134-2138 (2003b) https://doi.org/10.1021/es0113192
  12. Cho, M., Kim, H., Cho, S. H., and Yoon, J., Investigation of ozone reaction in river waters causing instantaneous ozone demand, Ozone Sci. Eng., 25, pp. 251-259 (2003c) https://doi.org/10.1080/01919510390481577
  13. Gyurek, L. L., Li, H., Belosevic, M., and Finch, G. R., Ozone inactivation kinetics of Cryptosporidium in phosphate buffer, J. Environ. Eng., 125, pp. 913-924 (1999) https://doi.org/10.1061/(ASCE)0733-9372(1999)125:10(913)
  14. Jenkins, M. B., Anguish, L. J., Bowman, D. D., Walker, M. J., and Ghiorse, W. C., Assessment of a dye permeability assay for determination of inactivation rates of Cryptosporidium parvum oocysts, Appl. Environ. Microbiol., 63, pp. 3844-3850 (1997)
  15. Rennecker, J., Marinas, B. J., Owens, J. H., and Rice, E. W., Inactivation of Cryptosporidium parvum oocysts with ozone, Wat. Res., 33, pp. 2481-2488 (1999) https://doi.org/10.1016/S0043-1354(99)00116-5
  16. Shin, G., Linden, K. G., Arrowood, M. J., and Sobsey, M. D., Low-pressure UV inactivation and DNA repair potential of Cryptosporidium parvum oocysts, Appl. Environ. Microbiol., 67, pp. 3029-3032 (2001)