Molecules and Cells

  • 제19권2호
  • /
  • Pages.228-231
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  • 2005
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

The Tobacco Ubiquitin-activating Enzymes NtE1A and NtE1B Are Induced by Tobacco Mosaic Virus, Wounding and Stress Hormones

  • Takizawa, Mari (Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo) ;
  • Goto, Akiko (Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo) ;
  • Watanabe, Yuichiro (Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo)
  • 투고 : 2004.11.02
  • 심사 : 2004.12.08
  • 발행 : 2005.04.30
⟨ 이전 논문 다음 논문 ⟩

초록

Recent characterization of several genes involved in plant defense responses suggested that ubiquitin-mediated protein degradation has a role in these responses. We isolated two cDNAs (NtUBA1 and NtUBA2) encoding ubiquitin-activating enzyme (E1) from Nicotiana tabacum cv. BY-2. The open reading frames of both encoded 1080 amino acids, corresponding to molecular masses of 120 kDa. The E1s and corresponding transcripts were upregulated by infection with tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV), and to a lesser extent by cucumber mosaic virus (CMV). Furthermore, they were also upregulated by wounding stress, and the plant hormones salicylic acid, jasmonic acid and the ethylene precursor, aminocyclopropane-1-carboxylic acid (ACC). Our findings support the idea that the ubiquitin-proteasome system plays a role in plant disease defenses.

키워드

과제정보

연구 과제번호 : Molecular Mechanisms of Plant-Pathogenic Microbe Interaction

연구 과제 주관 기관 : Ministry of Education, Culture, Sports, Science, and Technology, Japan

참고문헌

  1. Austin, M. J., Muskett, P., Kahn, K., Feys, B. J., Jones, J. D., et al. (2002) Regulatory role of SGT1 in early R gene-mediated plant defenses. Science 295, 2077-2080 https://doi.org/10.1126/science.1067747
  2. Azevedo, C., Sadanandom, A., Kitagawa, K., Freialdenhoven, A., Shirasu, K., et al. (2002) The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance. Science 295, 2073-2076 https://doi.org/10.1126/science.1067554
  3. Becker, F., Buschfeld, E., Schell, J., and Bachmair, A. (1993) Altered response to viral infection by tobacco plants perturbed in ubiquitin system. Plant J. 3, 875-881 https://doi.org/10.1111/j.1365-313X.1993.00875.x
  4. Dunigan, D. D., Dietzgen, R. G., Schoelz, J. E., and Zaitlin, M. (1988) Tobacco mosaic virus particles contain ubiquitinated coat protein subunits. Virology 165, 310-312 https://doi.org/10.1016/0042-6822(88)90691-5
  5. Haas, A. L., Warms, J. V., Hershko, A., and Rose, I. A. (1982) Ubiquitin-activating enzyme. Mechanism and role in proteinubiquitin conjugation. J. Biol. Chem. 257, 2543-2548
  6. Hatfield, P. M. and Vierstra, R. D. (1989) Analysis of the ubiquitin- dependent proteolytic pathway in wheat germ: isolation and characterization of the ubiquitin-activating enzyme (E1). Biochemistry 28, 735-742 https://doi.org/10.1021/bi00428a048
  7. Hatfield, P. M. and Vierstra, R. D. (1992) Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis. J. Biol. Chem. 267, 14799-14803
  8. Hatfield, P. M., Gosink, M. M., Carpenter, T. B., and Vierstra, R. D. (1997) The ubiquitin-activating enzyme (E1) gene family in Arabidopsis thaliana. Plant J. 11, 213-226 https://doi.org/10.1046/j.1365-313X.1997.11020213.x
  9. Hazelwood, D. and Zaitlin, M. (1990) Ubiquitinated conjugates are found in preparations of several plant viruses. Virology 177, 352-356 https://doi.org/10.1016/0042-6822(90)90490-I
  10. Jockusch, H. and Wiegand, C. (2003) Misfolded plant virus proteins: elicitors and targets of ubiquitylation. FEBS Lett. 545, 229-232 https://doi.org/10.1016/S0014-5793(03)00549-0
  11. Liu, Y., Schiff, M., Serino, G., Deng, X. W., and Dinesh-Kumar, S. P. (2002) Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to Tobacco mosaic virus. Plant Cell 14, 1483-1496 https://doi.org/10.1105/tpc.002493
  12. McGrath, J. P., Jentsch, S., and Varshavsky, A. (1991) UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme. EMBO J. 10, 227-236
  13. Peart, J. R., Lu, R., Sadanandom, A., Malcuit, I., Moffett, P., et al. (2002) Ubiquitin ligase-associated protein SGT1 is required for host and nonhost disease resistance in plants. Proc. Natl. Acad. Sci. USA 99, 10865-10869
  14. Reichel, C. and Beachy, R. N. (2000) Degradation of tobacco mosaic virus movement protein by the 26S proteasome. J. Virol. 74, 3330-3337 https://doi.org/10.1128/JVI.74.7.3330-3337.2000
  15. Saito, T., Meshi, T., Takamatsu, N., and Okada, Y. (1987) Coat protein gene sequence of tobacco mosaic virus encodes a host response determinant. Proc. Natl. Acad. Sci. USA 84, 6074-6077
  16. Vierstra, R. D. (2003) The ubiquitin/26S proteasome pathway, the complex last chapter in the life of many plant proteins. Trends Plant Sci. 8, 135-142 https://doi.org/10.1016/S1360-1385(03)00014-1