Neoparamoeba sp. Infection on Gills of Olive Flounder, Paralichthys olivaceus in Korea

  • Kim, Hyoung-Jun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Cho, Jae-Bum (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Lee, Mu-Kun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Huh, Min-Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Ki-Hong (Department of Aquatic Life Medicine, Pukyong National University)
  • 발행 : 20050800

초록

Amoebic gill disease of flounder, Paralichthys olivaceus was diagnosed at commerical culture facility in South Korea. The amoeba was identified as a species of the genus Neoparamoeba based on the morpholgical characteristics of trophozoites. Transmisson electron microscopy revealed the presence of a symbiotic organism, parasome in the cytoplasm and dense glycocalyx on the surface of the trophozoites. They lacked the boat-shaped microscales on the surface and contained numerous vacuoles and channels, mitochondria in the cytoplasm. Colonization of amoebae on gill tissue elicited extensive fusion and hyperplasia of gill lamella.

키워드

참고문헌

  1. Adams, M. and Nowak, B. F.: Distribution and structure of lesions in the gills of Atlantic salmon (Salmo salar L.) affected with amoebic gill disease. J. Fish Dis., 24: 535-542, 2001 https://doi.org/10.1046/j.1365-2761.2001.00330.x
  2. Adams, M. and Nowak, B. F.: Amoebic gill disease (AGD): sequential pathology in cultured Atlantic salmon, Salmo salar L. J. Fish Dis., 26:601-614,2003 https://doi.org/10.1046/j.1365-2761.2003.00496.x
  3. Dykova, I. and Novoa, B.: Comments on diagnosis of amoebic gill disease(AGD) in turbot, Scophthalmus maximus. Bull. Eur. Assoc. Fish Pathol., 21:40-44,2001
  4. Dykova, I., Figueras, A. and Novoa, B.: Amoebic gill infection of turbot, Scophthalmus maximus. Folia Parasitol., 42: 91-96, 1995
  5. Dykova, I., Figueras, A., Novoa, B. and Fernandez-Casal, J.: Paramoeba sp., an agent of amoebic gill disease of turbot Scophthalmus maximus. Dis. Aquat. Org., 33: 137-141, 1998 https://doi.org/10.3354/dao033137
  6. Dykova, I., Figueras, A. and Peric, Z.: Neop-aramoeba Page, 1987: light and electron microscopic observations on six strains of different origin. Dis. Aquat. Org., 43: 217-223,2000 https://doi.org/10.3354/dao043217
  7. Dykova, I., Fiala, I., Lom, J. and Lukes, J.: Perkin-siella amoebae-like endosymbionts of Neop-aramoeba spp., relatives of the kinetoplastid Ichthyobodo. Europ. J. Protistol., 39: 37-52, 2003 https://doi.org/10.1078/0932-4739-00901
  8. Dykova, I. Nowak, B. F., Crosbie, P. B. B., Fiala, I., Peckova, H., Adams, M. B., Machackova, B. and Dvorakova, H.: Neoparamoeba branchiphila n. sp., and related species of the genus Neoparamoeba Page, 1987: morphological and molecular characterization of selected strains. J. Fish Dis., 28: 49-64, 2005 https://doi.org/10.1111/j.1365-2761.2004.00600.x
  9. Hollande, A.: Identification du parasome (nebenkern) de Janickina pigmentifera a un symbionte {Perkinsella amoebae nov. gen. -nov. sp.) apparente aux flagelles kinetoplas-tidies. Protistologica, 16: 613-625, 1980
  10. Kent, M. L., Sawyer, T. K. and Hedrick, R. P.: Paramoeba pemaquidensis (Sarco-mastigophora: Paramoebidae) infestation of the gills of coho salmon Oncorhynchus kisutch reared in seawater. Dis. Aquat. Org., 5: 163-169,1988 https://doi.org/10.3354/dao005163
  11. Leiro, J., Paniaqua, E., Ortega, M., Parama, A., Fernandez, J. and Sanmartin, M. L.: An amoeba associated with gill disease in turbot, Scophthalmus maximus (L.). J. Fish Dis., 21: 281-288, 1998 https://doi.org/10.1046/j.1365-2761.1998.00104.x
  12. Munday, B. L., Foster, C. K., Roubal, F. R. and Lester, R. J. G.: Paramoebic gill infection and associated pathology of Atlantic salmon, Salmo salar, and rainbow trout, Salmo gairdneri in Tasmania, (ed. by Perkins F. O. and Cheng T. C.), Pathology in Marine Science. Academic Press, Lodon, UK, pp. 215-222, 1990
  13. Munday, B. L., Lange, K., Foster, C., Lester, R. T. G. and Handlinger, J.: Amoebic gill disease of sea-caged salmonids in Tasmania waters. Tasmanian Fish Res.,28: 14-19,1993
  14. Nash, G., Nash, M. and Schilotfeld, H. J.: Systemic amoebiasis in cultured European catfish, Sil-urus glanis L. J. Fish Dis., 11: 57-71,1988 https://doi.org/10.1111/j.1365-2761.1988.tb00523.x
  15. Noble, A. C, Herman, R. L., Noga, E. J. and Bullock, G. L.: Recurrent amoebic gill infestation in rainbow trout cultured in a semi-closed water recirculation system. J. Aquat. Anim. Health, 9: 64-69,1997 https://doi.org/10.1577/1548-8667(1997)009<0064:RAGIIR>2.3.CO;2
  16. Page, F. C.: Marine Gymnamoebae. Institute of Terrestrial Ecology, Culture Centre of Algae and Protozoa Cambridge, England, 54 pp, 1983
  17. Page, F. C: The classification of 'naked' amoebae of phylum Rhizopoda. Arch. Protistenkd., 133: 199-217,1987 https://doi.org/10.1016/S0003-9365(87)80053-2
  18. Roubal, F. R., Lester, R. J. G. and Foster, C. K.: Studies on cultured and gill-attached Paramoeba sp. (Gymnamoebae: Paramoe-bidae) and the cytopathology of paramoebic gill disease in Atlantic salmon, Salmo salar L., from Tasmania. J. Fish Dis., 12: 481-493, 1989 https://doi.org/10.1111/j.1365-2761.1989.tb00559.x
  19. Zilberg, D. and Munday, B. L.: Pathology of experimental gill disease in Atlantic salmon, Salmo salar L., and the effect of pre-mainte-nance of fish in sea water on the infection. J. Fish Dis., 23:401-407, 2000 https://doi.org/10.1046/j.1365-2761.2000.00252.x