Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Association of Aster Yellow Phytoplasma with Witches' Broom Disease of Ash (Fraxinus rhynchophylla Hence) in Korea

  • Han, Sang Sub (Division of Forest Science, College of Agriculture & Life Science, Chonbuk National University)
  • Received : 2005.02.18
  • Accepted : 2005.04.11
  • Published : 2005.04.30
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Abstract

Typical phytoplasma whiches' broom symptoms were observed in Ash (Fraxinus rhynchophylla Hence) in Korea. The symptoms of the disease were showing abnormally small leaves, shorted internodes and proliferation of shoots. Examination of fluorescent and electron microscopy of leaf midribs revealed numerous phytoplasma bodies localized in the phloem tube cells. The phytoplasmas were detected in all the symptomatic samples by the amplification with phytoplasma specific primer pair P1/P7 consistently, and the expected size was 1.8 kb. However, the phytoplasma DNA was not detected in healthy seedlings. Based on sequence analysis of amplified region, this phytoplasma has close homologies with eqilodium phyllody, mulberry dwarf, and aster yellow phytoplasmas, 99.95%, 99.79% and 99.78%, respectively, This phylogetic analysis indicates that ash witches' broom phytoplasma should be classified in the aster yellow group 16SrVI and clearly distinct from the ash yellow group 16SrVII.

Keywords

Acknowledgement

Supported by : Ministry of science & Technology, Korea Science & Engineering Foundation

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