Decolorization of Blue-Stain by Dual Culture of Blue Staining and Basidial Fungi

  • Pashenova, Natalia (Department of Physical and Chemical Biology and Biotechnology of Woody Plants, V.N. Sukachev Institute of Forest SB RAS) ;
  • Lee, Jong-Kyu (Division of Forest Resources, Kangwon National University) ;
  • Cho, Nam-Seok (School of Forest Resources, Chungbuk National University)
  • Received : 2004.10.04
  • Accepted : 2004.11.17
  • Published : 2005.03.25

Abstract

This study was performed to understand the interaction between Ophiostomataceae and basidiomycetes fungi during cultures, and whether the basidiomycetes fungi inhibit the growth and decolorize dark pigments of blue staining fungi. The conjoint cultivation was studied on 2% malt extract agar. The ability of basidial cultures to decolorize dark pigments of ophiostomatoid fungi was the main characteristics estimated during this study. More than half of basidial cultures were characterized by deadlock interaction with blue staining fungi. In the dual cultures, where basidial partners were presented by Agaricus bisporus(64), Laetiporus sulphureus(L01/89), Trametes versicolor(09) and unknown fungus(02), antagonism was found at the phase of primary contact of colonies. Replacement interaction resulted usually in decreasing dark colour of substrate was observed for 11 basidial cultures that were belonging mainly to white-rot fungi. Among them Abortiporus biennis(123), Antrodiella hoehnelii(S28/91), Bjerkandera fumosa (137), and Gleophyllum odoratum(124) were characterized by the absence of deadlock-phase: they began to grow over dark colonies of their partners just after primary contact. Basidiomycetes did not affect strongly the pigments of Ceratocystis spp. and Leptographium sibirica isolates, but completely decolorized colonies of Ophiostoma ips and to a smaller degree Ophiostoma minus. Antrodiella hoehnelii(S28/91), Bjerkandera fumosa(137), Gleophyllum odoratum(124) and Trametes versicolor(B18/91) cultures were found to be the most active in decreasing dark color of blue staining fungi colonies. The cultures were recommended for further development as agents of biopulping of wood chips and bio-control of blue stain in woods.

Keywords

Acknowledgement

Supported by : KOSEF

References

  1. Akhtar M., M. C. Attridge, G. C. Myers, and R. A. Blanchette. 1993. Biomechanical pulping of loblolly pine chips with selected white rot fungi. Holzforschung 47(1): 36-40 https://doi.org/10.1515/hfsg.1993.47.1.36
  2. Behrendt C. J. and R. A. Blanchette. 1997. Biological processing of pine logs for pulp and paper production with Phlebiopsis gigantea. Appl. Environ. Microbiol. 63(5): 1995-2000
  3. Behrendt C. J. and R. A. Blanchette. 2001. Biological control of blue stain in pulpwood: mechanisms of control used by Phlebiopsis gigantea. Holzforschung 55(3): 238-245 https://doi.org/10.1515/HF.2001.039
  4. Benko R. and B. Henningson. Mycoparasitism by some white rot fungi in culture. Intern. Res. Group Wood Preserv. Doe. No. IRG/WP/1304. 1986.-Stockholm, Sweden. pp. 1-5
  5. Croan S. and T. L. Highley. 1991. Biological control of the blue stain fungus Ceratocystis coerulescens with fungal anatagonists. Material and Organismen 25(4): 255-266
  6. Fischer K., M. Akhtar, R. A. Blanchette, T. A. Burnes, K. Messner, and T. K. Kirk. 1994. Reduction of resin content in wood chips during experimental biological pulping processes. Holzforschung 48(3): 285-290 https://doi.org/10.1515/hfsg.1994.48.4.285
  7. Jacobs K. and M. J. Wingfield. Leptographium species. Tree Pathogens, Insect Associates, and agents of Blue-Stain. 2001. St. Paul, Minnesota: APS Press. p.207
  8. Martinez-Inigo M. J., P. Immerzeel, A. Gutierrez, J. C. del Rio, and R. Sierra-Alvarez. 1999. Biodegradability of extractives in sapwood and heartwood from Scots pine by sapstain and white rot fungi. Holzforschung 53(3): 247-252 https://doi.org/10.1515/HF.1999.042
  9. Rayner A. D. M. and L. Boddy. 1988. Fungal communities in the decay of wood. Advances in Microbial Ecology 10: 115-166
  10. Samuels G. J. 1993. The case for distinguishing Ceraiocystis and Ophiostoma. In: Ceratocystis and Ophiostoma. Taxonomy, Ecology and Pathogenicity. St. Paul, Minnesota: APS Press. pp.15-20
  11. Zheng Y., N. R. Ruddick, and C. Breuil. 1995. Factors affecting the growth of Ophiostoma piceae on lodgepole pine heartwood. Material and Organismen 29(2): I05-117