Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Cloning and Expression of a Farnesyl Diphosphate Synthase in Centella asiatica (L.) Urban

  • Kim, Ok Tae (Department of Biology and Institute of Plant Resources, Chonnam National University) ;
  • Ahn, Jun Cheul (Department of Life Sciences, Seonam University) ;
  • Hwang, Sung Jin (Department of Food and Biotechnology, Dongshin University) ;
  • Hwang, Baik (Department of Biology and Institute of Plant Resources, Chonnam National University)
  • Received : 2004.11.06
  • Accepted : 2005.01.18
  • Published : 2005.04.30
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Abstract

A cDNA encoding farnesyl diphosphate synthase (FPS; EC2.5.1.1/EC2.5.1.10) was isolated from Centella asiacita (L.) Urban, using degenerate primers based on two highly conserved domains. A full-length cDNA clone was subsequently isolated by rapid amplification of cDNA ends (RACE) PCR. The sequence of the CaFPS (C. asiatica farnesyl diphosphate synthase) cDNA contains an open reading frame of 1029 nucleotides encoding 343 amino acids with a molecular mass of 39.6 kDa. The deduced CaFPS amino acid sequence exhibits 84, 79, and 72%, identity to the FPSs of Artemisia annua, Arabidopsis thaliana, and Oryza sativa, respectively. Southern blot analysis suggested that the C. asiatica genome contains only one FPS gene. An artificially expressed soluble form of the CaFPS was identified by SDS-PAGE. It had high specific activity and produced farnesyl diphosphate as the major isoprenoid.

Keywords

Acknowledgement

Supported by : Korea Research Foundation

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