Clinical and Experimental Reproductive Medicine

  • 제32권3호
  • /
  • Pages.269-277
  • /
  • 2005
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

쥐의 초기 난포 발달에 관여하는 Cell Size Growth 및 CCN Family 유전자에 관한 연구

Characterization of Genes Related to the Cell Size Growth and CCN Family According to the Early Folliculogenesis in the Mouse

  • 김경화 (포천중문의과대학교 생명과학전문대학원) ;
  • 박창은 (포천중문의과대학교 생명과학전문대학원) ;
  • 윤세진 (차병원 여성의학연구소) ;
  • 이경아 (포천중문의과대학교 생명과학전문대학원)
  • Kim, Kyeoung-Hwa (Graduate School of Life Science and Biotechnology, Pochon CHA University College of Medicine) ;
  • Park, Chang-Eun (Graduate School of Life Science and Biotechnology, Pochon CHA University College of Medicine) ;
  • Yoon, Se-Jin (Infertility Medical Center, CHA General Hospital) ;
  • Lee, Kyung-Ah (Graduate School of Life Science and Biotechnology, Pochon CHA University College of Medicine)
  • 발행 : 2005.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: Previously, we sought to compile a list of genes expressed during early folliculogenesis by using cDNA microarray to investigate follicular gene expression and changes during primordialprimary follicle transition and development of secondary follicles (Yoon et al., 2005). Among those genes, a group of genes related to the cell size growth was characterized during the ovarian development in the present study. Methods: We determined ovarian expression pattern of six genes related to the cell size growth (cyr61, emp1, fhl1, socs2, wig1 and wisp1) and extended into CCN family (${\underline{c}}onnective$ tissue growth factor/${\underline{c}}ysteine$-rich 61/${\underline{n}}ephroblastoma$-overexpressed), ctgf, nov, wisp2, wisp3, including cyr61 and wisp1 genes. Expression of mRNA and protein according to the ovarian developmental stage was evaluated by in situ hybridization, and/or semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), and immunohistochemistry, respectively. Results: Among 6 genes related to the cell size growth, cyr61 and wisp1 mRNA was detected only in oocytes in the postnatal day5 mouse ovaries. cyr61 mRNA expression was limited to the nucleolus of oocytes, while wisp1 was expressed in the cytoplasm and nucleolus of oocytes, except nucleus. cyr61 mRNA expression, however, was found in granulosa cells from secondary follicles. The rest 4 genes in the cell size growth group were detected in oocytes, granulosa and theca cells. Cyr61 and Wisp1 proteins were expressed in the oocyte cytoplasm from primordial follicle stage. Especially, Cyr61 protein was detected in pre-granulosa cells, Wisp1 protein was not. By using RT-PCR, we evaluated and decided that Cyr61 protein is produced by their own mRNA in pre-granulosa cells that was not detected by in situ hybridization. cyr61 and wisp1 genes are happen to be the CCN family members. The other members of CCN family were also studied, but their expression was detected in oocytes, granulose and theca cells. Conclusions: We firstly characterized the ovarian expression of genes related to the cell size growth and CCN family according to the early folliculogenesis. Cyr61 protein expression in the pre-granulosa cells is profound in meaning. Further functional analysis for cyr61 in early folliculogenesis is under investigation.

키워드

과제정보

연구 과제 주관 기관 : 한국학술진흥재단

참고문헌

  1. Hirshfield AN. Development of follicles in the mammalian ovary. Int Rev Cytol 1991; 124: 43-101 https://doi.org/10.1016/S0074-7696(08)61524-7
  2. McGee EA, Hsueh AJ. Initial and cyclic recruitment of ovarian follicles. Endocr Rev 2000; 21: 200-14. https://doi.org/10.1210/er.21.2.200
  3. Fortune JE, Cushman RA, Wahl CM, Kito S. The primordial to primary follicle transition. Mol Cell Endocrinol 2000; 163: 53-60 https://doi.org/10.1016/S0303-7207(99)00240-3
  4. Fortune JE. The early stages of follicular development: activation of primordial follicles and growth of preantral follicles. Anim Reprod Sci 2003; 78: 135-63 https://doi.org/10.1016/S0378-4320(03)00088-5
  5. 인쇄중: Yoon SJ, Kim KH, Chung HM, Choi DH, Lee WS, Cha KY, Lee KA. Gene expression profiling of early follicular development in primordial, primary and secondary follicles. Fertil Steril. In press 2005
  6. Eppig JJ, Wigglesworth K. Development of mouse and rat oocytes in chimeric reaggregated ovaries after interspecific exchange of somatic and germ cell components. Biol Reprod 2000; 63: 1014-23 https://doi.org/10.1095/biolreprod63.4.1014
  7. Wrenzycki C, Wells D, Herrmann D, Miller A, Oliver J, Tervit R, et al. Nuclear transfer protocol affects messenger RNA expression patterns in cloned bovine blastocysts. Biol Reprod 2001; 65: 309-17 https://doi.org/10.1095/biolreprod65.1.309
  8. Bork P. The modular architecture of a new family of growth regulators related to connective tissue growth factor. FEBS Lett 1993; 327: 125-30 https://doi.org/10.1016/0014-5793(93)80155-N
  9. Eppig JJ, Wigglesworth K, Pendola FL. The mammalian oocyte orchestrates the rate of ovarian follicular development. Proc Natl Acad Sci USA 2002 5; 99: 2890-4
  10. Cooper GM, Hausman RE. The cell a molecular approach. 3nd ed. Washington: ASM press; 2004
  11. Brigstock DR. The connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (CCN) family. Endocr Rev 1999; 20: 189-206 https://doi.org/10.1210/er.20.2.189
  12. Brigstock DR. The CCN family: a new stimulus package. J Endocrinol 2003; 178: 169-75 https://doi.org/10.1677/joe.0.1780169
  13. Perbal B. NOV (nephroblastoma overexpressed) and the CCN family of genes: structural and functional issues. Mol Pathol 2001; 54: 57-79 https://doi.org/10.1136/mp.54.2.57
  14. Lau LF, Lam SC. The CCN family of angiogenic regulators: the integrin connection. Exp Cell Res 1999 10; 248: 44-57
  15. Perbal B, Brigstock DR, Lau LF. Report on the second international workshop on the CCN family of genes. Mol Pathol 2003; 56: 80-5 https://doi.org/10.1136/mp.56.2.80
  16. Hwa V, Oh Y, Rosenfeld RG. The insulin-like growth factor-binding protein (IGFBP) superfamily. Endocr Rev 1999; 20: 761-87 https://doi.org/10.1210/er.20.6.761
  17. Mariani S, Basciani S, Arizzi M, Spera G, Gnessi L. PDGF and the testis. Trends Endocrinol Metab 2002; 13: 11-7 https://doi.org/10.1016/S1043-2760(01)00518-5
  18. Kim HS, Nagalla SR, Oh Y, Wilson E, Roberts CT Jr, Rosenfeld RG. Identification of a family of lowaffinity insulin-like growth factor binding proteins (IGFBPs): characterization of connective tissue growth factor as a member of the IGFBP superfamily. Proc Natl Acad Sci USA 1997; 94: 129811-29816
  19. Burren CP, Wilson EM, Hwa V, Oh Y, Rosenfeld RG. Binding properties and distribution of insulinlike growth factor binding protein-related protein 3 (IGFBP-rP3/NovH), an additional member of the IGFBP Superfamily. J Clin Endocrinol Metab 1999; 84: 1096-103 https://doi.org/10.1210/jc.84.3.1096
  20. Perbal B. The CCN family of genes: a brief history. Mol Pathol 2001; 54: 103-4 https://doi.org/10.1136/mp.54.2.103
  21. Monget P, Bondy C. Importance of the IGF system in early folliculogenesis. Mol Cell Endocrinol 2000; 163: 89-93 https://doi.org/10.1016/S0303-7207(99)00244-0
  22. Mo FE, Muntean AG, Chen CC, Stolz DB, Watkins SC, Lau LF. CYR61 (CCNl) is essential for placental development and vascular integrity. Mol Cell Biol 2002; 22: 8709-20 https://doi.org/10.1128/MCB.22.24.8709-8720.2002