Archives of Plastic Surgery

  • 제32권6호
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  • Pages.683-688
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  • 2005
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  • 2234-6163(pISSN)
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  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지

흰쥐 골격근의 허혈-재관류 손상후 생화학적 변화에 미치는 Melatonin의 효과

The Effect of Melatonin on Biochemical Changes after Ischemia-Reperfusion Injury of Rat Skeletal Muscle

  • 박혜준 (인제대학교 의과대학 일산백벽원 성형외과학교실) ;
  • 범진식 (이화여자대학교 의과대학 성형외과학교실)
  • Park, Hye June (Department of Plastic and Reconstructive Surgery, Ilsan Paik Hospital, Inje University College of Medicine) ;
  • Burm, Jin Sik (Department of Plastic and Reconstructive Surgery, Ewha Womans University College of Medicine)
  • 투고 : 2005.07.28
  • 발행 : 2005.11.10
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초록

The ischemia-reperfusion injury of the skeletal muscles is caused by generation of reactive oxygen during ischemia and reperfusion. Melatonin or N-Acetyl-5-methoxy- tryptamine is suggested to have antioxidant effects in several tissues. In present study, we examined the protective effect of melatonin in a rat hind limb ischemia-reperfusion injury. Dimethyl-sulfoxide(DMSO) was also tested for comparison. Ischemia was induced for 4 hours by vascular clamping and followed by 1 hour or 24 hours of reperfusion. Muscle injury was evaluated in 4 groups such as single laparotomy group(control), ischemia-reperfusion group, DMSO group, melatonin group. Eedema ratio and malondialdehyde(MDA) of muscle tissue and serum level of creatine kinase(CK), were measeured at the end of reperfusion. DMSO and melatonin group showed significant amelioration of edema and serum CK compared with ischemia-reperfusion group. The decreasing effect was more prominent in melatonin group. The muscle tissue MDA concentration is significantly lower in melatonin group than in ischemia-reperfusion group. The results show that melatonin prevents and improves ischemia-reperfusion injury more effectively in a rat hind limb than DMSO dose. Thus, clinically the melatonin may be used for a beneficial treatment of such injuries

키워드

참고문헌

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