Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Isolation of pathogenic Photobacterium damselae subsp. damselae from olive flounder, Paralichthys olivaceus

넙치, Paralichthys olivaceus에서 병원성 Photobacterium damselae subsp. damselae의 분리

  • Kwon, Mun-Gyeong (East Sea Fisheries Research Institute, National Fisheries Research & Development) ;
  • Park, Saung-Un (Finfish research center, East Sea Fisheries Research Institute, National Fisheries Research & Development) ;
  • Bang, Jong-Deuk (East Sea Fisheries Research Institute, National Fisheries Research & Development) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 권문경 (국립수산과학원 동해연구소) ;
  • 박상언 (국립수산과학원 동해연구소 어류연구센터) ;
  • 방종득 (국립수산과학원 동해연구소) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Published : 20051200
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Abstract

The isolates, which has caused considerable damage to the olive flounder farm located in the eastern coast of Korea showed 99% sequence homology in the comparison of 16s rRNA gene of P. damselae subsp. damselae ATCC 33539. The present P. damselae was identical to the biotype No.8 in Pedersen et al. (1997) and the same LPS protein pattern as P. damselac subsp. damselae ATCC 33539. The comparison of infection rates among present P. damselae and Vibrio spp. showed that isolated P. damselae was the highest, followed by V. anguillarium, V. harveyi. and V. ordalii.

최근 2년간 동해안 지역 넙치 양식장의 양식 넙치에 피해를 일으키는 P. damselae 균을 분리하였으며, 분리된 P. damselae의 16s rRNA 염기 서열은 P. damselae subsp. damselae ATCC 33539와 99%의 상동성을 나타내었다. 분리 균주는 Pedersen et al.(1997)의 biotype과 비교한 결과, biotype No 8과 동일하게 나타났으며, P. damselae subsp. damselae ATCC 33539의 LPS와 동일한 단백질 패턴을 나타내었다. 넙치의 병어로부터 P. damselae와 Vibrio 속 세균의 감염 상태를 조사한 결과, P. damselae가 가장 높은 감염율을 나타내었고, 그 다음으로 V. anguillarum, V. splendidus, V. harveyi와 V. ordalii순으로 감염율이 나타났다.

Keywords

References

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