Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

Biological Monitoring of Paint Handling Workers exposed to PAHs using Urinary 1-Hydroxypyrene

다핵방향족탄화수소류에 노출된 페인트 취급 근로자에서 요 중 1- Hydroxypyrene을 이용한 생물학적 모니터링

  • Lee, Jong-Seong (Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency) ;
  • Kim, Eun-A (Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency) ;
  • Lee, Yong-Hag (Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency) ;
  • Moon, Deog-Hwan (Institute of Industrial Medicine and Department of Occupational & Environmental Medicine, Paik Hospitul, Inje University) ;
  • Kim, Kwang-Jong (Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency)
  • 이종성 (한국산업안전공단 산업안전보건연구원) ;
  • 김은아 (한국산업안전공단 산업안전보건연구원) ;
  • 이용학 (한국산업안전공단 산업안전보건연구원) ;
  • 문덕환 (인제대학교 백병원 산업의학과 및 산업의학연구소) ;
  • 김광종 (한국산업안전공단 산업안전보건연구원)
  • Received : 2005.01.28
  • Accepted : 2005.07.27
  • Published : 2005.08.26
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Abstract

To investigate the exposure effect of polynuclear aromatic hydrocarbons (PAHs), we measured airborne total PAHs as an external dose, urinary 1-hydroxypyrene (1-OHP) as an internal dose of PAHs exposure, and analyzed the relationship between urinary 1-OHP concentration and PAHs exposure. The study population contained 44 workers in steel-pipe coating and paint manufacture industries. The airborne PAHs was obtained during survey day, and urine were sampled at the end of shift. Personal information on age, body weight, height, eniployment duration, smoking habit, and alcohol consumption was obtained by a structured questionnaire. Airborne PAHs were analyzed by the gas chromatograph with mass selective detector. Urinary 1-OHP levels were analyzed by the high performance liquid chromatograph with ultraviolet wavelength detector. For statistical estimation, t-test, ${\chi}^2$-test, analysis of variance, correlation analysis, arid regression analysis were executed by SPSS/PC (Windows version 10). The mean of environmental total PAHs was $87.8{\pm}7.81{\mu}g/m^3$. The mean concentration ($526.5{\pm}2.85{\mu}g/m^3$) of workers in steel-pipe coating industries using coal tar enamel was the higher than that ($17.5{\pm}3.36{\mu}g/m^3$) of workers in paint manufacture industries using coal tar paint. The mean of urinary 1-OHP concentration ($51.63{\pm}3.144{\mu}\;mol/mol$ creatinine) of workers in steel-pipe coating industries was the higher than that ($2.33{\pm}4.709{\mu}\;mol/mol$ creatinine) of workers in paint manufacture industries. The mean of urinary 1-OHP concentration of smokers was the higher than that of non-smokers. There was significant correlation between the urinary concentration of 1-OHP and the environmental concentration of PAHs (r=O.S48, p<0.001), pyrene(r=0.859, p<0.001), and urinary cotinine (r=0.324, p<0.05). The regression equation between the urinary concentration of 1-OHP in ${\mu}g/g$ creatinine($C_{1-OHP}$) and airborne concentration of PAHs (or pyrene) in ${\mu}g/m^3$ ($C_{PAHs}$ or Cpyrene) is: Log ($C_{1-OHP}$)=-0.650+0.889×Log($C_{PAHs}$), where $R^2=0.694$ and n=38 for p<0.001.Log ($C_{1-OHP}$)=1.087+0.707${\times}$Log(Cpyrene), where $R^2=0.713$ and n=38 for p<0.001. From the results of stepwise multiple regression analysis about 1-OHP, significant independents were total PAHs and urinary cotinine (adjusted $R^2=0.743$, p<0.001). In this study, there were significant correlation between the urinary concentration of 1-OHP and the airborne concentration of PAHs. The urinary 1-OHP was effective index as a biomarker of airborne PAHs in workplace. But it was influenced by non-occupational PAHs source, smoking.

Keywords

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