Toxicity Assessment of Biocide using Chlamydomonas reinhardtii

Chlamydomonas reinhardtii를 이용한 농약의 독성평가

  • Lee, Yong-Doo (Department of Environmental Engineering, Cheju National University) ;
  • Ko, In-Beom (Department of Environmental Engineering, Cheju National University) ;
  • Shin, Woo-Seok (Department of Environmental Engineering, Cheju National University)
  • 이용두 (제주대학교 환경공학과) ;
  • 고인범 (제주대학교 환경공학과) ;
  • 신우석 (제주대학교 환경공학과)
  • Received : 2005.02.07
  • Accepted : 2005.04.25
  • Published : 2005.07.30

Abstract

The average specific growth rate of C. reinhardtii (${\mu}$) was decreased with increase in biocide concentrations. The toxicity of biocides toward was as follows (in descending order of toxicities): herbicide 〉 pesticide 〉fungicide. $EC_{50}$ in each biocide was 0.0017 mg/L, 1.06 mg/L and 13.3 mg/L for herbicide, pesticide and fungicide respectively. When herbicide and pesticide were mixed, $EC_{50}$ was decreased by $2.7{\times}10^{-7}mg/L$. $EC_{50}$ in effective components of each biocide was 5.26 mg/L, 9.37 mg/L and 20.58 mg/L for herbicide, pesticide and fungicide respectively. Mixed main components of herbicide and pesticide caused to decrease by 3.10 mg/L.

Keywords

References

  1. 김은주, 이성규, 환경독성 평가를 위한 좀개 구리밥의 성장 저해시험법에 관한 연구, 한국환경독성학회지, 16, pp. 205-209 (2001)
  2. 신우석, 이용두, 고인법, Chlamydomonas reinhardtii를 이용한 농약의 독성평가, 대한상하수도학회.한국물환경학회 2004년 공동 추계학술발표회 논문집, p. 71 (2004)
  3. 이은경, Chlamydomonas reinhardtii의 생장에 미치는 biocides의 영향, 서울대학교 대학원 석사학위논문, p. 19 (1983)
  4. 이창균, Chlamydomonas reinhardtii의 생장에 미치는 환경오염원의 영향, 밀양대학교 대학원 석사학위논문, p. 9 (1990)
  5. Britz, M. L., Simonov, N. and Chun, U. H., Stablilization of Bioluminescence of Immobilized Photobacterium phosphoreum and Monitoring of Environmental Pollutants, J. Microbiol. Biotchnol., 7, pp. 242-249 (1997)
  6. Insecticide Factsheet, Imidacloprid, J. Pest. Reform, 21, p. 19 (2001)
  7. Mallak, F. P. and Brunker, R. L. Determination of the Toxicity of Selected Metalworking Fluid Preservatives by Use of the Microtox System and an in vitro Enzyme Assay, In Liu D. and Dutka(ed.), B. J. Toxicity Screening Procedures using Bacterials Systems, Marcel Dekker, Inc., New York, pp. 65-76 (1984)
  8. Patin, S. A., Pollution and the Biological Resources of the Oceans, Butter worth Scientific Press, Londen, pp. 80-109 (1982)
  9. Ramanathan, S., Ensor, M. and Daunert, S., Bacterial Biosensors for Monitoring Toxic Metals, TIBTECH., 15, pp. 500-506 (1997) https://doi.org/10.1016/S0167-7799(97)01120-7
  10. Rochaix, J. D., Mayfield, S., Goldschmit, C. M. and Erickson, J., Molecular biology of Chlamydomas : In Plant Molecular Biology., IRL PRESS, Oxford, pp. 253-25 (1988)
  11. Sager, R. and S. Granick, Ann, N, Y, Acad., Sci., 56, pp. 831-838 (1953)
  12. van Dyk, T. K., Majarian, W. R., konstantinov, K. B., Young, R. M., Dhurjati, P. S. and Larossa, R. A., Rapid and Sensitive Pollutant Detection by Induction of Heat Shock Gene-Bioluminescence Gene Fusions, Appl. Environ. Microbiol., 60, pp. 1414-1420 (1994)