한국산림과학회지 (Journal of Korean Society of Forest Science)

한국산림과학회 (Korean Society of Forest Science)
권호 표지

포플러의 Phytochrome B 유전자 분리 및 특성구명

Isolation and characterization of Phytochrome B gene in Poplar

  • Kang, Hoduck (Department of Forest Resources, Dongguk University) ;
  • Lee, Keum-Young (Department of Forest Resources, Dongguk University) ;
  • Kang, Sang-Gu (School of Biotechnology, Yeungnam University) ;
  • Bae, Han-hong (USDA-ARS, Plant Science Institute, Beltsville Agricultural Research Center)
  • 투고 : 2005.02.28
  • 심사 : 2005.08.02
  • 발행 : 2005.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

다양한 광조건에서 식물의 생장조절에 관여하는 광수용체인 phytochrome B(PhyB) 유전자를 교잡종 포플러 수항1호에서 분리하였다. 염기서열분석 결과, PhyB cDNA는 길이가 3,456bp 이었으며 1,156개의 아미노산으로 구성된 단백질을 암호화하고 있는 것으로 나타났다. PhyB 단백질은 아미노산 수준에서 Populus balsamfera PhyB1과 98%의 높은 상동성을 나타내었다. Northern blot 분석 결과, PhyB 유전자는 광조건에서는 높은 수준으로 발현되지만, 암조건에서는 발현되지 않는 것으로 나타났다. 본 연구의 결과들을 종합하여 볼 때 PhyB는 빛에 의하여 발현이 유도되며 광수용체 역할을 하는 것으로 여겨진다.

Phytochrome B (PhyB) gene, which is a photoreceptor that controls plant growth under various light conditions, was cloned from Chinese hybrid poplar 'Soohang 1'. Nucleotide sequence and deduced amino acid sequences PhyB cDNA of 'Soohang' is consisted with 3,456 nucleotides and 1,156 amino acids. The cloned PhyB fragment showed 98% homology of amino acid sequences with Populus balsamifera PhyB1. According to Northern blot analysis. PhyB was up-regulated by light, while PhyB transcript was not detected under dark condition. According to this study, the cloned PhyB is induced by light and functions as photoreceptor.

키워드

과제정보

연구 과제 주관 기관 : 한국학술진흥재단

참고문헌

  1. Choi, G., H. Yi, J. Lee, Y.K. Kwon, M.S. Soh, B. Shin, Z. Luka, T.R. Hahn, and P.S. Song. 1999. Phytochrome signalling is mediated through nucleoside diphosphate kinase 2. Nature 401 : 610-613 https://doi.org/10.1038/44176
  2. Christensen, A.H. and P.H. Quail. 1989. Structure and expression of a maize phytochrome encoding gene. Gene 85 : 381-390 https://doi.org/10.1016/0378-1119(89)90431-9
  3. Clack, T., S. Mathews, and R.A. Sharrock. 1994. The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYO and PHYE. Plant Mol. BioI. 25 : 413-427 https://doi.org/10.1007/BF00043870
  4. Elich T.D. and J. Chory. 1997. Phytochrome: if it looks and smells like a histidine kinase, is it a histidine kinase? Cell 91 : 713-716 https://doi.org/10.1016/S0092-8674(00)80458-4
  5. Friedrich B., J.M. Andrew, A.K., Steve, and F. Masaki. 1997. Phytochrome-induced intercellular signalling activates cab: luciferase gene expression. Plant 12 : 839-849 https://doi.org/10.1046/j.1365-313X.1997.12040839.x
  6. Furuya M. 1993. Phytochromes-their molecular species, gene families and functions. Ann. Rev. Plant Physiol. Plant Mol. Biol. 44: 617-645 https://doi.org/10.1146/annurev.pp.44.060193.003153
  7. Furuya M. and P.S. Song. 1994. Assembly and properties of holophytochrome. In: Kendrick R.E., G.H.M. Kronenberg, eds. Photomorphogenesis in plants. Dordrecht/Boston/London: Kluwer Academic Publishers. pp 105-140
  8. Grimm R., D. Gast and W. Rudiger. 1989. Characterization of a protein kinase activity associated with phytochrome from etiolated oat seedlings. Planta 178 : 199-206 https://doi.org/10.1007/BF00393195
  9. Hershey, H.P., R.F. Barker, K.B. Idler, J.L. Lissemore and P.H. Quail. 1985. Analysis of cloned cDNA and genomic sequences for phytochrome: complete amino acid sequences for two gene products expressed in etiolated Avena. Nucleic Acids Res. 13 : 8543-8559 https://doi.org/10.1093/nar/13.23.8543
  10. Heyer, A. and C. Gatz. 1992. Isolation and characterization of a cDNA-clone coding for potato type B phytochrome. Plant Mol. Biol. 20 : 589-600 https://doi.org/10.1007/BF00046444
  11. Howe, G.T., WP. Kackett, G.R. Fumier, and R.E. Klevorn. 1995. Photoperiodic responses of a northern and southern ecotype of black cottonwood. Physiol. Plant. 93: 695-708 https://doi.org/10.1111/j.1399-3054.1995.tb05119.x
  12. Howe G.T., G.M. Gardner, G.M. Gardner, W.P. Hackett, and G.R. Fumier. 1996. Phytochrome control of short-day induced bud set in black cottonwood. Physiol. Plant. 97 : 95-103 https://doi.org/10.1111/j.1399-3054.1996.tb00484.x
  13. Kendrick, R.E. and G.H.M. Kronenberg. 1994. Photomorphogenesis in Plants. Dordrecht: Kluwer Academic. p 420
  14. Kolukisaoglu, H.U., B. Braun, W.F. Martin and H.A. Schneider-Poetsch. 1993. Mosses do express conventional, distantly B-type-related phytochromes. Phytochrome of Physcomitrella patens (Hedw.). FEBS Lett. 334 : 95-100 https://doi.org/10.1016/0014-5793(93)81689-W
  15. Mathews, S. and R.A. Sharrock. 1997. Phytochrome gene diversity. Plant Cell Environ. 20 : 666-671 https://doi.org/10.1046/j.1365-3040.1997.d01-117.x
  16. Murray, M.G and WF. Thompson. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8 : 4321-4325 https://doi.org/10.1093/nar/8.19.4321
  17. Okamuro, J.K., B.G.W den Boer and K.D. lofuku. 1993. Regulation of Arabidopsis flower development. Plant Cell 5: 1183-1193 https://doi.org/10.1105/tpc.5.10.1183
  18. Quail P.H. 1991. Phytochrome: A light-activated molecular switch that regulates plant gene expression. Ann. Rev. Gen. 25 : 389-409 https://doi.org/10.1146/annurev.ge.25.120191.002133
  19. Quail, P.H. 1997. An emerging molecular map of the phytochromes. Plant Cell Environ. 20 : 657-665 https://doi.org/10.1046/j.1365-3040.1997.d01-108.x
  20. Reed, J.W, P. Nagpal, D.S. Poole., M. Furuya, and J. Chory. 1993. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell. 5 : 147-157 https://doi.org/10.1105/tpc.5.2.147
  21. Sharrock, R.A. and P.H. Quail. 1989. Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev. 3 : 1735-1757 https://doi.org/10.1101/gad.3.11.1735
  22. Smith, H. 1995. Physiological and ecological function within the phytochrome family. Ann. Rev. Plant Physiol. Plant Mol. Biol. 46 : 289-315 https://doi.org/10.1146/annurev.pp.46.060195.001445