Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Nonlinear Adsorption Isotherm of Single and Multi-Components of 2'-Deoxyribonucleosides

2'-deoxyribonucleosides의 단일 및 다성분계의 비선형 흡착평형식

  • Jin, Long Mei (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Han, Soon Koo (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Choi, Dae-Ki (Clean Technology Research Center, Korea Institute of Science and Technology) ;
  • Row, Kyung Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
  • 김룡매 (인하대학교 화학공학과, 초정밀생물분리기술연구센터) ;
  • 한순구 (인하대학교 화학공학과, 초정밀생물분리기술연구센터) ;
  • 최대기 (한국과학기술연구원 청정기술연구센터) ;
  • 노경호 (인하대학교 화학공학과, 초정밀생물분리기술연구센터)
  • Received : 2004.05.31
  • Accepted : 2004.09.30
  • Published : 2005.04.30
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Abstract

Reversed-phase high-performance liquid chromatography (RP-HPLC) was used to determine the equilibrium isotherm of single and multi-components of dUrd(2'-deoxyuridine), dGuo(2'-deoxyguanosine), and dAdo(2'-deoxyadenosine) of 2'-deoxyribonucleosides by dynamic method. The composition of mobile phase was 90/10 vol.% (water/MeOH). With an increase in the injection volumes, the retention times were shorter and the peak shapes were triangle-shaped, so Langmuir-type isotherm was assumed. The Langmuir adsorption parameters were estimated by PIM (pulsed-input method), and the competitive Langmuir adsorption isotherm was further utilized. For the sample of the dUrd and dGuo whose retention times were relatively short, the agreement of between the calculated value and experimental data was fairly good in both single and multi-components, but for the dAdo, the last eluting component, some deviations were caused by non-linear and non-ideal properties.

2'-deoxyribonucleosides중에서 dUrd(2'-deoxyuridine), dGuo(2'-deoxyguanosine), dAdo(2'-deoxyadenosine)의 단일 및 다성분계의 흡착평형식을 동적방법으로 구하기 위해 물과 메탄올(90/10, vol%)을 이동상으로 하는 역상 액체 크로마토그래피를 사용하였다. 시료의 주입부피가 증가함에 따라서 체류시간이 짧아지고 피크가 삼각형 모양을 보여서, Langmuir 흡착평형식으로 가정하였다. PIM (pulsed-input method)에 의해서 Langmuir 흡착평형식의 매개변수를 구하고, 경쟁적인 다성분계의 흡착평형식을 사용하였다. 체류시간이 짧은 dUrd와 dGuo의 경우에서는 단일 및 다성분계에서 실험값과 계산값이 잘 일치하였지만, 가장 늦게 용출되는 dAdo에서는 비이상 비선형적인 특성으로 인해서 약간의 오차가 있었다.

Keywords

Acknowledgement

Supported by : 인하대학교

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