The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Kung-kyung-IlHo-jeon on Induced Apoptosis in Human Cervical Careinoma HeLa Cells

궁경1호전(宮頸1號煎)이 자궁경부암세포(子宮頸部癌細胞)(HeLa Cell)에 미치는 영향(影響)

  • Kang, Young-Keum (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Choe, Chang-Min (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Cho, Han-Back (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Yoo, Sim-Keun (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University)
  • 강영금 (원광대학교 한의과대학 부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 부인과학교실) ;
  • 유심근 (원광대학교 한의과대학 부인과학교실)
  • Received : 2004.07.28
  • Accepted : 2004.08.07
  • Published : 2005.02.28
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Abstract

To address the ability of Kung-Kyung-Ilho-Jeon(KK) to induce cell death, we investigated the effect of KK on cell viability. Forty-eight hours later, loss of viability occurred following KK exposure in a dose-dependent manner. The treatment of KK, a commonly used herb formulation in Korea and China, caused a decrease in cell viability. KK also resulted in apoptotic morphology a brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining, and reduction of cell volume. Our results show that KK induces caspase-3 and -9 activation in a time-dependent manner. In addtion, the translocation of cytochrome c release into cytoplasm has been observed under the presence of $5mg/m{\ell}$ KK. The subsequent loss of mitochondria membrane potential is collapsed by the addition of KK. Our immunoblotting data show that PARP, a well known caspase-3 and -6 substrate, is cleaved by KK. We show that a pro-apoptotic protein, Bax is increased in the presence of KK but that the amount of Bcl-2 is not changed. We suggest that Bax, a critical protein which can regulate channel of mitochondria to release cytochrome c, is a key protein in KK-induced apoptosis of Hela human cervical carcinoma cells

Keywords

References

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