Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Development of an ELISA for the Detection of Fenazaquin Residues in Fruits

  • Lee, Jae-Koo (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Kim, Yun-Jung (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Lee, Eun-Young (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Kim, Dae-Kyu (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Kyung, Kee-Sung (Department of Agricultural Chemistry, Chungbuk National University)
  • Published : 2005.03.31
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Abstract

To develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the residues of the acaricide fenazaquin, five haptens were synthesized and assessed. A competitive indirect format was used with polyclonal antibodies. Under an optimized condition using the selected rabbit C antiserum, an $IC_{50}$ of $96.97\;ng{\cdot}ml^{-1}$, the detection range of $14.9{\sim}631\;ng{\cdot}ml^{-1}$, and the lowest detection limit of $8\;ng{\cdot}ml^{-1}$ were obtained. Some structurally related compounds of practical use showed low crossreactivities to the antibody. Highest cross-reactivity observed with hapten IV indicates that the antiserum C recognizes very well quinazoline ring, 4-tert-butylphenyl, and an adequate length of spacer arm. The length of spacer arm affected recognition of quinazoline ring and 4-tert-butylphenyl moieties. When applied to apple and pear, recoveries were within acceptable ranges of $93.18{\sim}104.77%$ (n = 4) and $79.40{\sim}111.95%$ (n = 4), respectively.

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