Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Nitrate Uptakes by Microorganisms Isolated from the Soils of Greenhouse

  • Cho, Kwang-Hyun (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Lee, Gyeong-Ja (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Ahn, Hae-Jin (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Kim, Young-Kee (Department of Agricultural Chemistry, Chungbuk National University)
  • Published : 2005.03.31
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Abstract

Salinity of soils in greenhouse has been increased by massive application of fertilizers. Nitrogen fertilizer was most popular, and thus nitrate became the majority of soil salinity. Accumulation of nitrate led to deleterious effects on the growth and development of crops and vegetables. Microbial strains able to utilize nitrate and thus remove excess nitrate from farm land soils were isolated from 15 different soils of greenhouses and plastic film houses. Four strains able to grow in medium containing 50 mM $KNO_3$ were isolated, among which only E0461 showed high capacity of nitrate uptake. Nitrate uptake by E0461 was dependent on culture medium and was increased by addition of tryptone and peptone. Although E0461 was able to grow without tryptone and peptone, growth was slow, and no nitrate uptake was observed. Nitrate appeared to facilitate E0461 growth in the presence of tryptone and peptone. Through kinetic analysis, nitrate uptake was measured at various concentrations of nitrate, and half-life was calculated. Nitrate concentration decreased with increasing incubation period, and plot between half-lives and initial concentrations of nitrate fitted to single exponential function. These results suggest one major factor plays an important role in microbial nitrate uptake.

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References

  1. J. Plant Nutr. v.27 Effects of nitrogen and phosphorus fertilization on plant growth and nitrate accumulation in vegetables Wang, Z.;Li, S. https://doi.org/10.1081/PLN-120028877
  2. Nature v.368 Costal eutrophication near the Mississippi river delta Turner, R.U.;Rabalals, N.N. https://doi.org/10.1038/368619a0
  3. Plant Cell Physiol. v.33 Distinct cellular and organismic responses to salt stress Adams, P.;Thomas, J.C.;Vernon, D.M.;Bohnert, H.J.;Jensen, R.G.
  4. Annu. Rev. Plant Physiol. Plant Mol. Biol. v.51 Plant cellular and molecular responses to high salinity Hasegawa, P.M.;Bressan, R.A.;Zhu, J.K.;Bohnert, H.J. https://doi.org/10.1146/annurev.arplant.51.1.463
  5. Nitrogen absorption by plant roots;Nitrogen Nutrition in Higher Plants Glass, A.D.M.;Siddiqi, M.Y.;Srivastava, H.S.(ed.);Singh, R.P.(ed.)
  6. Biochim. Biophys. Acta v.1465 Nitrate transporters in plants: structure, function and regulation Forde, B.G. https://doi.org/10.1016/S0005-2736(00)00140-1
  7. Kor. J. Soil Sci. Fert. v.29 Effect of salt accumulation on the germination and growth of lettuce (Lactuca sativa, L.) Kang, B.G.;Jeong, I.M.;Min, K.B.;Kim, J.J.
  8. Kor. J. Soil Sci. Fert. v.37 Estimation of optimum application rate of nitrogen fertilizer based on soil nitrate concentration for tomato cultivation in plastic film house Kang, S.S.;Hong, S.D.
  9. Kor. J. Soil Sci. Fert. v.30 Chemical characteristics of plastic film house soils in Chungbuk area Kang, B.G.;Jeong, I.M.;Kim, J.J.;Hong, S.D.;Min, K.B.
  10. Fertil. Res. v.33 Leaching of nitrate, calcium and magnesium under maize cultivation in an oxisol in Togo Poss, R.;Saragoni, H. https://doi.org/10.1007/BF01051167
  11. RDA J. Agri. Sci. v.35 The effect of several desalting methods applied to vinyl house soils Hwang, S.W.;Kim, Y.S.;Yeon, B.Y.;Lee, Y.J.;Park, Y.D.
  12. Kor. J. Soil Sci. Fert. v.30 Vertical distribution of bulk density and salts in a plastic film house soil Kim, P.J.;Lee, D.K.;Chung, D.Y.
  13. J. Hydrol. v.221 Large scale modelling of groundwater contamination from nitrogen leaching Refsgaard, J.C.;Thorsen, M.;Jensen, J.B.;Kleeschulte, S.;Hansen, S. https://doi.org/10.1016/S0022-1694(99)00081-5
  14. Nutr. Cycl. Agroecosyst. v.46 Nitrate leaching in temperate agroecosystems: sources, factors and mitigating strategies Di, H.J.;Cameron, K.C.
  15. Water SA v.25 Denitrification by heterotrophic bacteria during activated sludge treatment Drysdale, G.D.;Kasan, H.C.;Bux, F.
  16. Appl. Microbiol. Biotechnol. v.49 Removal of organic pollutants and of nitrate from wastewater from the dairy industry by denitrification Zayed, G.;Winter, J. https://doi.org/10.1007/s002530051200
  17. Kor. J. Biotechnol. Bioeng. v.18 Isolation and characterization of denitrification bacteria Cha, W.S.;Choi, H.I.;Lee, D.B.;Cha, J.M.
  18. Methods of Soil and Plant Analysis NIAST
  19. Kor. J. Soil Sci. Fert. v.37 Effect of nitrogen content of irrigation water and soil EC on lettuce growth Lee, G.J.;Kang, B.G.;Kim, H.J.;Park, S.G.
  20. Curr. Opin. Plant Biol. v.2 Nitrate regulation of metabolism and growth Stitt, M. https://doi.org/10.1016/S1369-5266(99)80033-8
  21. J. Biol. Chem. v.270 The Escherichia coli P signal transduction protein is activated upon binding 2-ketoglutarate and ATP Kamberov, E.S.;Atkinson, M.R.;Ninfa, A.J. https://doi.org/10.1074/jbc.270.30.17797
  22. Plant J. v.18 Molecular and functional regulation of two $NO_3$ uptake systems by N- and C-status of Arabidopsis plants Lejay, L.;Tillard, P.;Lepetie, M.;Olive, F.D.;Filleur, S.;Daniel-Vedele, F.;Gojon, A. https://doi.org/10.1046/j.1365-313X.1999.00480.x
  23. Adv. Microbiol. Physiol. v.39 Nitrate assimilation by bacteria Lin, J.T.;Stewart, V.