Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Expression of Antioxidant Isoenzyme Genes in Rice under Salt Stress and Effects of Jasmonic Acid and ${\gamma}$-Radiation

  • Kim, Jin-Hong (Division of Radiation Application Research, Korea Atomic Energy Research Institute (KAERI)) ;
  • Chung, Byung-Yeoup (Division of Radiation Application Research, Korea Atomic Energy Research Institute (KAERI)) ;
  • Baek, Myung-Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute (KAERI)) ;
  • Wi, Seung-Gon (Division of Radiation Application Research, Korea Atomic Energy Research Institute (KAERI)) ;
  • Yang, Dae-Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute (KAERI)) ;
  • Lee, Myung-Chul (National Institute of Agricultural Biotechnology, R.D.A.) ;
  • Kim, Jae-Sung (Division of Radiation Application Research, Korea Atomic Energy Research Institute (KAERI))
  • Published : 2005.03.31
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Abstract

Analysis of chlorophyll (Chl) fluorescence implicated treatment of 40 mM NaCl decreased maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm), actual quantum yield of PSII (${\Phi}_{PSII}$), and photochemical quenching (qP) in rice, but increased non-photochemical quenching (NPQ). Decreases in Fv/Fm, ${\Phi}_{PSII}$, and qP were significantly alleviated by $30\;{\mu}M$ jasmonic acid (JA), while NPQ increase was enhanced. Transcription levels of antioxidant isoenzyme genes were differentially modulated by NaCl treatment. Expression of cCuZn-SOD2 gene increased, while those of cAPXb, CATb, and CATc genes decreased. JA prevented salt-induced decrease of pCuZn-SOD gene expression, but caused greater decrease in mRNA levels of cAPXa and Chl_tAPX genes. Investigation of vacuolar $Na^+/H^+$ exchanger (NHX2) and 1-pyrroline-5-carboxylate synthetase (P5CS) gene expressions revealed transcription level of NHX2 gene was increased by JA, regardless of NaCl presence, while that of P5CS gene slightly increased only in co-presence of JA and NaCl. Unlike JA, ${\gamma}$-radiation rarely affected expressions of antioxidant isoenzyme, NHX2, and P5CS genes, except for increase in mRNA level of Chl_tAPX and decrease in that of pCuZn-SOD. These results demonstrate enhanced salt-tolerance in JA-treated rice seedlings may be partly due to high transcription levels of pCuZn-SOD, NHX2, and P5CS genes under salt stress.

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References

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