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A Combination of PG490 and Lipopolysaccharide Induce Apoptosis through Activation of Casapase-3 and Downregulation of cIAP1 and XIAP in Human Astroglioma Cell

  • Lee, Tae-Jin (Department of Immunology and Chronic Disease Research Center and Institute for Medical Science, School of Medicine, Keimyung University) ;
  • Woo, Kyung-Jin (Department of Immunology and Chronic Disease Research Center and Institute for Medical Science, School of Medicine, Keimyung University) ;
  • Park, Jong-Wook (Department of Immunology and Chronic Disease Research Center and Institute for Medical Science, School of Medicine, Keimyung University) ;
  • Kwon, Taeg-Kyu (Department of Immunology and Chronic Disease Research Center and Institute for Medical Science, School of Medicine, Keimyung University)
  • Published : 2005.06.30
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Abstract

Background: Malignant gliomas are the most common primary tumors in the central nervous system. Methods: We investigated the combined effect of PG490 and LPS on the induction of the apoptotic pathway in human astroglioma cells. Results: Treatment of U87 cells with combination of 50nM of PG490 and $50{\mu}g/ml$ of LPS resulted in increased internucleosomal DNA fragmentation, cleavage of PLC-${\gamma}1$, and downregulation of cIAP1 and XIAP. The combination of LPS and PG490 treatment-induced apoptosis is mediated through the activation of caspase, which is inhibited by the caspase inhibitor, z-VAD-fmk. Also, release of cytochrome c was found in PG490 and LPS-cotreated U87 cell. Conclusion: Taken together, combination of PG490 and LPS appears to be a potent inducer of apoptosis in astrogliaoma cells, and might have some benefit in the treatment of glioma patients.

Keywords

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