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The Korean Association of Immunobiologists (대한면역학회)
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Immune Regulatory Function of Dendritic Cells Expressing Indoleamine 2,3-Dioxygenase in Orally Tolerance to Type II Collagen-induced Animal Model

제2형 콜라겐 경구관용 유도 동물모델에서 수지상 세포의 Indoleamine 2,3-dioxygenase의 의존성 관절염 항원 특이 T세포 증식반응 제어 연구

  • Park, Min-Jung (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • Min, So-Youn (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • Park, Kyoung-Su (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • Cho, Mi-La (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • CHo, Young-Gyu (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • Min, Jun-Ki (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • Yoon, Chong-Hyeon (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • Park, Sung-Hwa (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
  • Kim, Ho-Youn (The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea)
  • 박민정 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 민소연 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 박경수 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 조미라 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 조영규 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 민준기 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 윤종현 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 박성환 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
  • 김호연 (가톨릭대학교 의과학연구원 류마티스연구센터)
  • Published : 2005.12.30
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Abstract

Background: Immune regulatory dendritic cells (DCs) play an important role in maintaining self-tolerance. Recent evidences demonstrate that DCs expressing indoleamine 2,3-dioxygenase (IDO), which is involved in tryptophan catabolism, play an important role in immunoregulation and tolerance and induce T cell apoptosis. This study was devised to examine the role of IDO in the oral tolerance induction in collagen-induced arthritis (CIA) mouse model. Methods: Beginning 2 weeks before immunization, CII was fed six times to DBA/1 mice and the effect on arthritis was assessed. In tolerized mice, $CD11c^+$ DCs were isolated and stimulated with CII, IFN-${\gamma}$, and LPS with or without IDO inhibitor, 1-methyl-DL-tryptophan (1-MT) and IDO expression by $CD11c^+$ DCs was analyzed using FACS and RT-PCR. The expression of IDO, MHC II, CD80, and CD86 by $CD11c^+$ DCs were examined using confocal microscopy. Regulatory effect of $CD11c^+$ DCs on Ag-specific T cell proliferative response to CII was examined by mixed lymphocyte reaction (MLR) with or without 1-MT. Results: The proportion of IDO-expressing $CD11c^+$ DCs was slightly higher in tolerized mice than in CIA mice and significantly increased after stimulation with CII, IFN-${\gamma}$, and LPS in an IDO-dependent manner. On confocal microscopic examination, the expression of IDO was higher and those of MHC II and CD86 were lower in CD11c + DCs from tolerized mice compared to those from CIA mice. On MLR, $CD11c^+$ DCs from tolerized mice inhibited T cell proliferative response to CII in an IDO-dependent manner. Conclusion: Enhanced IDO expression by $CD11c^+$ DCs from tolerized mice may contribute to the regulation of proliferative response of CII-reactive T cells and could be involved in the induction of oral tolerance to CII.

Keywords

References

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