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Preferential Expression of IgA Isotype Switching-associated Transcripts in Mouse Intestinal Lymphoid Tissues

마우스 내장 림프조직에서 우세하게 발현되는 IgA Isotype Switching 관련 전사체의 분석

  • Chae, Byung-Chul (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Chun, Sung-Ki (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Seo, Goo-Young (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Kim, Hyun-A (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Kim, Pyeung-Hyeun (Department of Microbiology, College of Natural Sciences, Kangwon National University)
  • 채병철 (강원대학교 자연대학 미생물학과) ;
  • 전성기 (강원대학교 자연대학 미생물학과) ;
  • 서구영 (강원대학교 자연대학 미생물학과) ;
  • 김현아 (강원대학교 자연대학 미생물학과) ;
  • 김평현 (강원대학교 자연대학 미생물학과)
  • Published : 2005.12.30
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Abstract

Background: Transforming growth factor-${\beta}$ (TGF-${\beta}1$) directs class switch recombination (CSR) to IgA isotype, which is a predominant antibody in mucosal surfaces. Although IgA is preferentially committed in mucosal lymphoid tissues, it is not definitely established whether hallmarks of IgA CSR such as IgA germ-line transcripts (GLT ${\alpha}$), post-switch transcripts (PST ${\alpha}$) and circle transcripts (CT ${\alpha}$) are readily expressed in such tissues. Therefore, we compared the expression of these transcripts among mouse Peyer's patches (PP), mesenteric lymph nodes (MLN), and spleen. Methods: Levels of GLTs, PSTs and CTs were measured by RT-PCR in isolated PPs, MLNs and spleen cells. Results: GLT ${\alpha}$ and PST ${\alpha}$ were well expressed in PP and MLN cells but in spleen cells. Similar patterns were observed in the expression of GL ${\gamma}$2b and PST ${\gamma}$2b. On the other hand, these transcripts were only inducible in spleen cells upon stimulated with LPS and TGF-${\beta}1$. In addition, CT${\alpha}$ and CT${\gamma}$2b were detected in PP cells. Conclusion: PP B cells readily express IgA GLT, PST, and CT. Overall expression patterns of these transcripts were similar in MLN cells. Thus, these results suggest that microenvironment of PP and MLN influences spontaneous IgA CSR, which lacks in systemic lymphoid tissues such as spleen.

Keywords

References

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