Food Science and Biotechnology

  • 제14권6호
  • /
  • Pages.854-859
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  • 2005
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  • 1226-7708(pISSN)
  • /
  • 2092-6456(eISSN)
한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Effect of Low Doses of Genistein and Equol on Protein Expression Profile in MCF-7 Cells

  • Kim, Jang-Hoon (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Lim, Hyun-Ae (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Lee, Jeong-Soon (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Sung, Mi-Kyung (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Kim, Young-Kyoon (Department of Forestry Products, Kookmin University) ;
  • Yu, Ri-Na (Department of Food and Nutrition, University of Ulsan) ;
  • Kim, Jong-Sang (Department of Animal Science and Biotechnology, Kyungpook National University)
  • 발행 : 2005.12.31
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초록

Although action modes of equol and genistein have been extensively studied, their precise roles in tumor cells remain elusive. To address possible effects of these compounds on protein expression in mammary tumor cells, proteins modulated in MCF-7 mammary tumor cells when incubated in absence and presence of 10 uM equol or genistein were identified through 2-dimensional gel electrophoresis, MALDI-TOF MS/MS, and NCBInr database search using Mascot software. Most proteins differentially expressed in MCF-7 cells after treatment with 10 uM genistein or equol were identified as being the same. Exposure to both compounds caused decreased cellular expression of RNA-binding protein regulatory subunit and oncogene DJ1 tubulin beta-1 chain, and increased expression of heterogeneous ribonucleoproteins F and L, KH-type splicing regulatory protein, and translation elongation factor EF-Tu precursor. Genistein and equol at dose used in this study showed common action mechanism.

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