Assurance on the Genotoxicological Safety of Fermented Vegetables Pasteurized by Gamma Irradiation

  • Yook, Hong-Sun (Department of Food and Nutrition, Chungnam National University) ;
  • Byun, Myung-Woo (Department of Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Song, Hyun-Pa (Department of Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Lee, Ju-Woon (Department of Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Kim, Kwan-Soo (Greenpia Tech Co.) ;
  • Kim, Kwang-Hoon (Greenpia Tech Co.) ;
  • Lee, Ho-Joon (Korea Food Research Institute) ;
  • Kim, Dong-Ho (Department of Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute)
  • 발행 : 2005.02.28

초록

The genotoxicological safety of fermented vegetables pasteurized by gamma irradiation was examined to consider the possibility of the application of irradiation for extending of fermented vegetables. A fermented vegetable was irradiated at 20 kGy to assure its toxicological safety even at a high dose of radiation. The Ames test with Salmonella typhimurium (TA98, TA100, TA1535, TA1537) and Escherchia coli (WP2), and the chromosomal aberration test in Chinese hamster lung (CHL) cells were performed. In vivo micronucleus test were conducted in mouse bone marrow cells. With or without metabolic activation, negative results were obtained in the Ames test and the chromosomal aberration test. In the micronucleus test, there was no enhancement in the formation of micronucleus, and there were no such significant differences between the irradiated and non-irradiated samples. The observed results indicated that, a level of 20 kGy of gamma irradiation on the fermented vegetable did not bring about any genotoxic effects under the described experimental conditions.

키워드

참고문헌

  1. Food Control v.15 Isolation of enteric pathogens in the fermentation process of Kimchi (Korean fermented vegetables) and its radicidation by gamma irradiation Kim, D.H.;Song, H.P.;Yook, H.S.;Ryu, Y.K.;Byun, M.W. https://doi.org/10.1016/S0956-7135(03)00119-1
  2. Radiat. Phys. Chem. v.69 Nutritional, physiological, physicochemical and sensory stability of gamma irradiated Kimchi (Korean fermented vegetables) Song, H.P.;Kim, D.H.;Yook, H.S.;Kim, M.R.;Kim, K.S.;Byun, M.W. https://doi.org/10.1016/S0969-806X(03)00329-3
  3. Food Sci. Biotechnol. v.13 Effect of the salt concentration on the radiation sensitivity of some fermentative microorganisms inhabiting salted and fermented food Kim, D.H.;Song, H.P.;Choi, I.D.;Kim, B.K.;Cheong, M.Y.;Byun, M.W.
  4. Food Sci. Biotechnol. v.10 Changes in microbiological and general qualities in gamma irradiated Doenjang (fermented soybean paste) Byun, M.W.;Kim, D.H.;Yook, H.S.;Cha, B.S.;Kim, J.O.
  5. High dose irradiation: Wholesomeness of food irradiated with doses above 10 kGy. WHO Technical Report Series 890 World Health Organization
  6. J. Food Quality v.13 Food irradiation: Benefits and concerns Thayer, D.W. https://doi.org/10.1111/j.1745-4557.1990.tb00014.x
  7. Mutat. Res. v.436 ICH-Hannonized guidances on genotoxicity testing of phamaceuticals: evolution, reasoning and impact Muller, L.;Kikuchi, Y.;Probest, G.;Schechtman, L.;Shimada, H.;Sofuni, T.;Tweats, D. https://doi.org/10.1016/S1383-5742(99)00004-6
  8. Guidelines for Testing of Chemicals OECD
  9. Mutat. Res. v.113 Revised methods for the Salmonella mutagenicity test Maron, D.M.;Ames, B.N. https://doi.org/10.1016/0165-1161(83)90010-9
  10. Gann Monograph on Cancer Res. v.27 Chromosomal aberration tests in vitro as a primary screening tool for environmental mtagens and/or carcinogens Ishidate, M. Jr.;Sofuni, T.;Yoshikawa, K.
  11. Assays for the detection of chemically induced chromosome damage in cultured mammalian cells;Mutagenicity testing a practical approach Dean, B.J.;Danford, N.;Venitt, S.(ed.);Parry, J.M.(ed.)
  12. Gann Monograph on Cancer Res. v.61 A new cell line derived from newborn Chinese hamster lung tissue Koyama, H.;Utakoji, T.;Ono, T.
  13. The bone marrow micronucleus test;Handbook of mutagenicity test procedures Heddle, J.A.;Stuart, E.;Salarnone, M.F.;Kilbey, B.J.(ed.);Legator, M.(ed.);Nichols, W.(ed.);Ramel, C.(ed.)
  14. Guideline for the testing of chemicals. Mammalian erythrocyte micronucleus test. Guideline 474 OECD
  15. SAS Users Guide SAS Institute, Inc.
  16. Mutat. Res. v.31 Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test Ames, B.N.;McCann, J.;Yamasaki, E. https://doi.org/10.1016/0165-1161(75)90046-1
  17. Mutat. Res. v.455 The bacterial tryptophan reverse mutation assay with Escherichia coli WP2 Kristien, M.;Edward, S.R. https://doi.org/10.1016/S0027-5107(00)00076-2
  18. Nat. Genet. v.15 A breakpoint map of recurrent chromosomal rearrangements in human neoplasia Mitelman, F.;Mertens, F.;Johansson, B. https://doi.org/10.1038/ng0497supp-417
  19. Guideline for the testing of chemicals;vitro mammalian chromosome aberration test. Guideline 4733. OECD
  20. The micronucleus test for cytogenetic analysis. Vol. 4;Chemical mutagenesis, Principles and Methods for their Detection Schmid, W.;Hollaender, A.(ed.)
  21. Mutat. Res. v.31 The micronucleus test Schmid, W. https://doi.org/10.1016/0165-1161(75)90058-8
  22. Mutat. Res. v.412 Genetic toxicological testing of some plant flavonoids by the micronucleus test Sahu, R.K.;Basu, R.;Sharma, A.
  23. J. Food Hyg. Safety v.15 no.4 Genotoxicological safety on water-soluble fraction of gamma irradiated Korean soybean fermentation foods Yook, H.S.;Lee, E.M.;Kim, D.H.;Lee, K.H.;Lee, H.J.;Lee, Y.N.;Byun, M.W.