International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Antioxidant Property of Vitamin C - in Comparison with Vitamin B1

  • Seo, Du-Kyo (Department of Biochemistry, College of Dentistry, Kyung Hee University) ;
  • Kim, Jeong-Hee (Department of Biochemistry, College of Dentistry, Kyung Hee University)
  • Published : 2005.12.31
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Abstract

Various aspects of antioxidant activity in vitamin C were evaluated in this study. Relatively high level of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was detected in vitamin C, but not in non-antioxidative vitamin, vitamin B1. Vitamin C also reduced the production of lipid peroxidation in Chinese hamster lung fibroblast (V79-4) cells with $IC_{50}$ value of $4{\mu}g/ml$. Vitamin B1 showed comparable reduction in lipid peroxidation products ($IC_{50}$ value was about $10{\mu}g/ml$). It was shown that vitamin C also dose-dependently enhanced the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in V79-4 cells, and these effects were not observed in vitamin Bl-treated cells. Our data suggest that well-known antioxidant vitamin C involved in direct activation of SOD, CAT and GPX.

Keywords

References

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