Patterns of Antimicrobial Resistance and Detection of mecA Gene from Methicillin Resistant Staphylococcus aureus Isolated from Healthcare Facilities and U.S. Military Hospital in Korea

  • Sin Chin-Su (Department of Biomedical Laboratory Science, Graduate School of Health and Environment, Yonsei University) ;
  • Lee Gyu-Sang (College of Health Science, Graduate School of Health and Environment, Yonsei University) ;
  • Lim Kwan-Hun (College of Health Science, Graduate School of Health and Environment, Yonsei University) ;
  • Kim Jong-Bae (Department of College of Health Science, Graduate School of Health and Environment, Yonsei University, College of Health Science, Yonsei University)
  • Published : 2005.12.01

Abstract

A total of 108 strains of MRSA (Methicillin-resistant Staphylococcus aureus) clinical isolates was collected from $121^{st}$ general hospital (U.S. military hospital), Korean healthcare facility from January to March in 2005 and Wonju Christian hospital in 1999. Antimicrobial susceptibility test by Vitek System and MIC test using oxacillin and cephalothin stripes by E-test were executed. PCR based detection of mecA gene was performed on the all of MRSA clinical isolates, too. MRSA clinical isolates were characterized with antimicrobial resistance patterns, PCR based detection of mecA gene and validation of the multiplex PCR strategy of SCCmec among clinical isolates.

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