Microsporum canis에 대한 5가지 항진균제와 맥반석 가루의 항진균 효과

Antifungal Activity of 5 Antifungal Agents and Granitic Powder on Microsporum canis

  • 강태형 (전남대학교 수의과대학) ;
  • 이정치 (서정대학 애완동물과) ;
  • 원영호 (전남대학교 의과대학 피부과학교실) ;
  • 오석일 (진도군 진돗개 시험연구소) ;
  • 이정길 (전남대학교 수의과대학) ;
  • 이채용 (전남대학교 수의과대학)
  • Kang Tae-hyung (College of Veterinary Medicine, Chonnam National University) ;
  • Lee Jeong-chi (Department of Veterinary nurse and pets sciences, Seojeong College) ;
  • Won Young-ho (Department of Dermatology, Chonnam National University) ;
  • Oh Seok-il (The Jindo Research Center) ;
  • Lee Chung-gil (College of Veterinary Medicine, Chonnam National University) ;
  • Lee Chai-yong (College of Veterinary Medicine, Chonnam National University)
  • 발행 : 2005.12.01

초록

The aim of this study was to determine tile inhibitory effect of granitic powder against Microsporum canis. Fourteen strains of M. canis isolated from dgs and cats with fungal dermatitis and two strains isolated from humans were used in this study. The in vitro antifungal activities of granitic powder and 5 commercialized antifungal agents (terbinafine, itraconazole, ketoconazole, griseofulvin and fluconazole) were compared. The antifungal effect was measured by the broth microdilution method and was expressed as the minimal inhibitory concentration (MIC). The MIC value of the granitic powder was ranged from 31.3 to 250mg/ml. Terbinafine showed the lowest MIC value among the 5 commercial antifungal agents $(0.0078-0.125{\mu}g/ml)$, while fluconazole showed the highest MIC values $(125-1,000{\mu}g/ml)$. The MIC range of itraconazole, griseofuvin and ketoconazole were $0.125-0.5{\mu}g/ml\;0.625-5{\mu}g/ml$ and $10-40{\mu}g/ml$ respectively. The Geometric mean(GM) MIC values of terbinafine and ketoconazole against M. canis isolated from human were $0.0078{\mu}g/ml\;and\;10{\mu}g/ml$, respectively, while the GM MIC values of these agents against M. canis isolated from animals were $0.063{\mu}g/ml\;and\;31.4{\mu}g/ml$, respectively. Other antifungal agents did not show any significant differences in antifungal activity against M. canis of animal or human origin. Although granitic powder was shown to have antifungal activity, it was much lower than that of the 5 commercialized antifungal agents.

키워드

참고문헌

  1. Barry AL, Brown SD. Fluconazole disk diffusion procedure for detennining susceptibility of Candida species. J Clin Microbiol 1996; 34: 2154-2157
  2. Chen SC, O'Donnell ML, Gordon S, Gibert GL. Antifungal susceptibility testing using the E test: comparison with the broth macrodilution technique. J Antimicrob Chemother 1996; 37: 265-273 https://doi.org/10.1093/jac/37.2.265
  3. Cormican MG, Pfaller MA. Standardization of antifungal susceptibility testing. J Antimicrob Chemother 1996; 38: 561-578
  4. Espinel-Ingroff A, Dawson K, Pfaller M, Anasissie E, Breslin B, Dixon D, Fothergill A, Paetznick V, Peter J, Rinaldi M, Walsh T. Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi. Antimicrob Agents Chemother 1995; 39: 314-319 https://doi.org/10.1128/AAC.39.2.314
  5. Gerorgii A, Korting HC. Antifungal susceptibility testing with dermatophytes. Mycoses 1991; 34: 193-199 https://doi.org/10.1111/j.1439-0507.1991.tb00643.x
  6. Granade TC, Artis WM. Antimycotic susceptibility testing of dermatophytes in microcultures with a standardized fragmented mycelial inoculum. Antimicrob Agents Chemother 1980; 17: 725-729 https://doi.org/10.1128/AAC.17.4.725
  7. Hazen KC. Fungicidal versus fungistatic activity of terbinafine and itraconazole: an in vitro comparison. J Am Acad Dermatol 1998; 38: 37-41 https://doi.org/10.1016/S0190-9622(98)70482-7
  8. Kazutoshi S, Walter F, Coulson JS, Wollman MW, Tsunehiro A, Toshiaki O, Shiro N. Histopathology of cryptococcosis and other fungal infections in patients with acquired immuno-deficiency syndrome. Int J Infect Dis 2001; 5: 78-85 https://doi.org/10.1016/S1201-9712(01)90030-X
  9. Kirk SM, Callister SM, Lim LC, Schell RF. Rapid susceptibility testing of Candida albicans by flow cytometry. J Clin Microbiol 1997; 35: 358-363
  10. Korting HC, Rosenkranz S. In vitro susceptibility of dematophytes from Munich to griseofulvin, miconazole and Ketoconazole. Mycoses 1990; 33: 136-139
  11. Kotnik T, Kozuh Erzen N, Kuzner J, Drobnic-Kosorok M. Terbinafine hydrochloride treatment of Microsporum canis experimentally-induced ringworm in cats. Vet Microbiol. 2001; 83: 161-168 https://doi.org/10.1016/S0378-1135(01)00412-6
  12. May JL, King A, Warren CA. Fluconazole disc diffusion testing for the routine laboratory. J Antimicrob Chemother 1997; 40: 511-516 https://doi.org/10.1093/jac/40.4.511
  13. Mizoguchi J, Hokao R, Sano J, Kagiyama N, Imamichi T. An outbreak of Trichophyton mentagrophytes infection in a rat breeding stock and its successful control. Jikken Dobutsu 1986; 35: 125-130
  14. Mock M, Monod M, Baudraz-Rosselet F, Panizzon RG. Tinea capitis dermatophytes: susceptibility to antifungal drugs tested in vitro and in vivo. Dermatology 1998; 197: 361-367 https://doi.org/10.1159/000018032
  15. National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing for yeasts: proposed standard M27-P. National Committee for Clinical Laboratory Standards. Villanova, Pa, 1992
  16. Niewerth M, Splanemann V, Korting HC, Ring J, Abeck D. Antimicrobial susceptibility testing of dermatophytes-comparison of the agar macrodilution and broth microdilution tests. Chemotherapy 1998; 44: 31-35
  17. Nimura K, Niwano Y, Ishiduka S, Fukumoto R. Comparison of in vitro antifungal activities of topical antimycotics launched in 1990s in Japan. Int J Antimicrob Agents 2001; 18: 173-178 https://doi.org/10.1016/S0924-8579(01)00365-X
  18. Peyron F, Favel A, Guiraud-Dauriac H, EI Mzibri M, Chastin C, Dumenil G, Regli P. Evaluation of a flow cytofluorometric method for rapid determination of amphotericin B susceptibility of yeast isolates. Antimicrob Agents Chemother 1997; 41: 1537-1540
  19. Puccini S, Valdre A, Papini R, Mancianti F. In vitro susceptibility to antimycotics of Microsporum canis isolates from cats. J Am Vet Med Assoc 1992; 201: 1375-1377
  20. Pujol I, Guarro J, Llop C, Soler L, Fernandez-Ballart J. Comparison study of broth macrodilution and microdilution antifungal susceptibility tests for the filamentous fungi. Antimicrob Agents Chemother 1996; 40: 2106-2110
  21. Rambali B, Fernandez JA, Van Nuffel L, Woestenborghs F, Baert L, Massart DL, Odds FC. Susceptibility testing of pathogenic fungi with itraconazole: a process analysis of test variables. J Antimicrob Chemother 2001; 48: 163-177 https://doi.org/10.1093/jac/48.2.163
  22. Scott DW, Miller WH, Griffin CE. Fungal skin disease. In: Muller & Kirk's small animal dermatology, 6th ed. Philadelphia: WB Saunders, 2001: 336-422
  23. Sparkes AH, Gruffydd-Jones TJ, Shaw SE, Wright AI, Stokes CR. Epidemiological and diagnostic features of canine and feline dermatophytosis in the United Kingdom from 1956 to 1991. Vet Rec 1993; 133: 57-61 https://doi.org/10.1136/vr.133.3.57
  24. 김도원, 오수희, 서순봉. Microsporum canis 감염증의 만연상태. 대한피부학회지 1983; 21: 695-702
  25. 김영욱, 최원필. 국내 동물원 동물의 피부사상균 및 Mycoflora의 분포. 한국수의공중보건학회지 2002; 26: 13-21
  26. 동의학연구소. 동의보감. 탕액편: 약으로 쓰는 돌. 서울: 이공출판사, 1994: 2835-2849
  27. 변동길, 서치균, 송준영, 남용진, 신현철. 수종 백선균에 대한 Griseofulvin의 항균력에 관하여. 대한피부과학회지 1965; 4: 31-36
  28. 서순봉,김도원,김재복. 우리나라에서 분리된 Microsporum canis의 교배형. 대한피부과학회지 1983; 21: 557-561
  29. 신재은, 성충현,김두. 개와 고양이에서 분리한 피부사상균에 대한 항진균제의 감수성시험. 한국임상수의학회지 2001; 18: 1-6
  30. 이헌준,전무형,김교준,김덕환,최원필. 개와 고양이의 피부사상균 보균실태조사. 대한수의사회지 1986; 22: 45-51
  31. 이헌준,최원필. 고양이와 개에서 분리한 Microsporum canis의 교배형. 대한수의학회지 1998; 28: 111-113
  32. 임소정,오강희,박진희,최원필. 소 피부사상균증의 발생 상황 및 분리균의 약제 감수성. 한국수의공중보건학회지 2002: 26: 23-29
  33. 임채형. 실험적 소포자균증 감염 기니픽에서의 Terbinafine의 효능 연구. 서울대학교 대학원 석사학위논문,1997
  34. 전의식,서순봉. 피부진균종의 각종 항진균제에 대한 감수성상. 대한피부과학회지 1979; 17: 221-227
  35. 최원필,윤성웅,송동준,이춘식,김영은,박철정. Microsporum canis에 의한 개의 피부사상균증 및 개,고양이의 피부사상균의 보균상황. 대한수의학회지 1993; 33: 235-239
  36. 최원필,주진숙. 항진균제에 대한 피부사상균의 감수성. 한국수의공중보건학회지 1998; 22: 201-205
  37. 한기옥,최원필. 개 피부병 유래의 Microsporum canis의 항진균제 감수성. 대한수의학회지 2001; 41: 173-176
  38. 황진연. 맥반석과 황토의 특성과 활용. 한국광물학회 창립 10주년 기념 심포지움 논문집 1997; 89-99