Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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The Prevalence of Mycoplasma hyopneumoniae Antigens in Pneumonic Lungs and Serum Antibodies of Slaughtered Pigs in Jeju

제주지역 도축돼지의 페렴병변에서 Mycoplasma hyopneumoniae 항원 및 혈중 항체 조사 연구

  • Kim Seung-Il (Department of Veterinary Medicine, Cheju National University) ;
  • Yang Hyoung-Seok (Department of Veterinary Medicine, Cheju National University) ;
  • Kim Jae-Hoon (Department of Veterinary Medicine, Cheju National University) ;
  • Bae Jong-Hee (Department of Veterinary Medicine, Cheju National University)
  • Published : 2005.12.01
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Abstract

Enzootic pneumonia caused by Mycoplasma hyopneumoniae is responsible for major economic losses in pig herds of world wide. Mycoplasma hyopneumoniae can also act as a primary pathogen of porcine respiratory disease complex followed by bacterial or viral infection. This study was carried out to investigate the prevalence of mycoplasmal pneumonia of slaughtered pigs in Jeju for two years. The lungs and sera of 214 cases were examined for gross and microscopic lesions of the lungs, immunohistichemistry test for Mycoplasma hyopneumoniae antigen and enzyme-linked immunohistichemistry assay (ELISA) for serum antibody titer. Pulmonary consolidation was observed in the lungs of 163 pigs $(76.1\%)$ with average gross lesion score of $6.0\%$., Bronchointerstitial pneumonia was most frequently observed $(78.5\%)$. The incidence of pulmonary consolidation was decreased in vaccinated pigs compared to that of non-vaccinated pigs. The rate of consolidation in the lungs was significantly decreased in the vaccinated pigs (P<0.05). Antigen of Mycoplasma hyopneumoniae was identified by immunohistichemistry test in the lungs of 174 pigs $(81.3\%)$. ELISh antibodies to Mycoplasma hyopneumoniae were detected in 154 pigs $(72.0\%)$. These results showed the prevalence of swine pneumonia and the incidence of Mycoplasma hyopneumoniae in slaughtered pigs of Jeiu province. We expect that these results would be helpful for the control of swine mycoplasmal pneumonia and porcine respiratory disease complex in Jeju.

Keywords

References

  1. Armstrong CH, Freeman MJ, Sands-Freeman L, Ropez-Osuna M, Young T, Runnels LJ. Comparison of the Enzyme-linked Immunosorbent Assay and Indirect Hemagglutination and Complement Fixation Tests for Detecting Antibodies to Mycoplasma hyopneumoniae. Can J Comp Med 1983; 47: 464-470
  2. Bolske G, Strandberg ML, Bergstrom K, Johannson KE. Species-specific antigens of Mycoplasma hyopneumoniae and cross-reaction with other porcine mycoplasma. Curr Microbiol 1987; 15: 233-239 https://doi.org/10.1007/BF01577537
  3. Doster AR, Lin BC. Identification of Mycoplasma hyopneumoniae in formalin-fixed porcine lung, using an indirect immunoperoxidase method. Am J Vet Res 1988; 49: 1719-1721
  4. Goodwin RFW, Pomeroy AP, Whittlestone P. Production of enzootic pneumonia in pigs with a mycoplasma. Vet Rec 1965; 77: 1247-1249
  5. Halbur PG, Paul PS, Janke BH. Viral contributors to the porcine respiratory disease complex. Proc Am Assoc Sw Pract 1993; 24: 343-350
  6. Jubb KVF, Kennedy PC, Palmer N. Inflammation of the lungs. In: Pathology of domestic animals Vol 2, 4th ed. San Diego: Academic press. 1993: 589-613
  7. Kristensen B, Nicolet J, Wanner M, de Weck AL. Cell-mediated and humoral immune response in swine after vaccination and natural infection with Mycoplasma hyopneumoniae. Am J Vet Res 1981; 42: 784-788
  8. Livingston CW, Stair EL, Underdahl NR, Mebus CA. Pathogenesis of Mycoplasmal Pneumonia in Swine. Am J Vet Res 1972; 33: 2249-2258
  9. Morris CR, Gardner IA, Hietala SK, Carpenter TE, Anderson RJ, Parker KM. Persistence of passively acquired antibodies to Mycoplasma hyopneumoniae in a swine herd. Prev Vet Med 1994; 21: 29-41 https://doi.org/10.1016/0167-5877(94)90030-2
  10. Morrison RB, Pijoan C, Hilley HD, Rapp V. Microorganisms associated with pneumonia in slaughter weight swine. Can J Comp Med 1985; 49: 129-137
  11. Piffer IA, Ross RF. Effect of age on susceptibility of pigs to Mycoplasma hyopneumoniae pneumonia. Am J Vet Res 1984; 45: 478-481
  12. Pointon AM, Sloane M. An abattoir survey of the prevalence of lesions of enzootic pneumonia of pigs in south Australia. Aust Vet J 1984; 58: 16-17 https://doi.org/10.1111/j.1751-0813.1982.tb00571.x
  13. Ross RF, Whittlestone P. Recovery of, identification of and serological response to porcine mycoplasmas. In: Methods in Mycoplasmology Vol 2. New York: Academic press. 1983: 240-255
  14. Schatzmann E, Keller H, Grest P, Lorenz D, Burri W. Field study with a vaccine against enzootic pneumonia of swine. Schweiz Arch Tierheilkd 1996; 138: 483-489
  15. Sheldrake RF, Gardner IA, Saunders MM, Romalis LF. Serum antibody response to Mycoplasma hyopneumoniae measured by enzyme-linked immunosorbent assay after experimental and natural infection of pigs. Aust Vet J 1990; 67: 39-42 https://doi.org/10.1111/j.1751-0813.1990.tb07691.x
  16. Sorensen V, Barford K, Feld NC. Evaluation of a monoclonal blocking ELISA and IHA for Mycoplasma hyopneumoniae in SPF-pig herds. Vet Rec 1992; 130: 488-490 https://doi.org/10.1136/vr.130.22.488
  17. Straw BE, D'Allaire S, Mengeling WL, Taylor DJ. Diseases of the respiratory system. In: Disease of swine, 8th ed. Ames: Iowa state university press. 1999: 913-940
  18. 김혜권, 김은미, 문형준, 김태융, 임종성, 이양호, 박봉균. 혈청학적조사를 통한 한국 양돈장에서의 Mycoplasma hyopneumoniae 감염 시점에 분석. 대한수의학회지 2004; 44: 587-591
  19. 류영수, 박최규, 김로미, 이창희, 최상호, 김승일, 배종희. 제주지역에 대한 돼지 주요 전염병의 혈청학적 역학조사. 대한수의학회지 1997; 37: 765-772
  20. 박응복, 임창형, 이준섭. 도축장에서의 폐충성 폐렴과 마이코플라즈마 폐렴의 병리조직학적 비교. 서울대학교 수의대 논문집 1977; 2: 43-54
  21. 이석규, 한정희, 김준영, 김현주. 양돈장의 사양 및 위생관리에 따른 출하돈에서의 폐렴발생. 대한수의학회지 1998; 38: 751-755
  22. 조광현, 최정수, 김봉환. 영남지방 도축돈의 Mycoplasma 폐렴조사 및 분리균에 대한 약제 감수성. 대한수의학회지 1999; 39: 96-103