Preventive Nutrition and Food Science

  • 제10권4호
  • /
  • Pages.353-356
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  • 2005
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  • 2287-1098(pISSN)
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  • 2287-8602(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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The Effects of Boron on the Proliferation of Osteoblastic and Neuroblastoma Cells

  • Choi, Hye-Sook (College of Pharmacy Sungkyunkwan University) ;
  • Hang, Do (College of Pharmacy Sungkyunkwan University) ;
  • Choi, Mi-Kyeong (Department of Human Nutrition & Food Science, Chungwoon University) ;
  • Lee, Sung-Ryul (College of Pharmacy Sungkyunkwan University) ;
  • Pyo, Suhkneung (College of Pharmacy Sungkyunkwan University) ;
  • Son, Eun-Wha (Department of Pharmacognosy Material Development Samcheok National University) ;
  • Kim, Mi-Hyun (Department of Food & Nutrition, Samcheok National University)
  • 발행 : 2005.12.01
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초록

It has been recently reported that boron affects bone metabolism in humans and animals. In this study we examined whether boron affects the proliferation on various cell types, MG-63, HOS, Raw 264.7 and SK-N-SH. When treated with different concentrations of boron $(1,\;10,\;100{\mu}M)$ for 24 and 48 hr, the proliferation of MG-63 cells was enhanced at $10{\mu}M\;(p<0.05)$, for 24 hr. In HOS cells, boron had no effect on cell proliferation at 24 or 48 hr. In addition, treatment of pre-osteoclastic cells (Raw 264.7) with 1, 10, $100{\mu}M$ boron resulted in no effect on cell proliferation. Proliferation of neuronal cells (SK-N-SH) was enhanced by boron in a concentration dependent manner at low concentrations (0.1, 0.5, $1{\mu}M$). Besides proliferation activity, boron has an effect on the enhancement of NO production in SK-N-SH cells in a concentration-dependent manner. These studies showed that boron enhances proliferation of osteoblastic cells (especially MG-63), depending upon the concentration of boron. These results also provide further evidence of the positive effects of boron in neuronal disease.

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참고문헌

  1. Riggs BL, Melton LJ. 1986. Medical progress: Involutional osteoporosis. N Engl J Med 314: 1676-1686 https://doi.org/10.1056/NEJM198606263142605
  2. Prockop DJ. 1971. Role of iron in the synthesis of collagen in connective tissue. Fed Proc 30: 984-990
  3. Calhoun NR, Smith JC, Becker KL. 1974. The role of zinc in bone metabolism. Clin Orthop 10: 212-234
  4. Opsahl W, Zeronian H, Ellison M, Lewis D, Rucker RB, Riggins RS. 1982. Role of copper in collagen cross-linking and its influence on selected mechanical properties of chick bone and tendon. J Nutr 112: 708-716 https://doi.org/10.1093/jn/112.4.708
  5. Amstrong TA, Spears JW. 2001. Effect of dietary boron on growth performance, calcium and phosphorus metabolism, and bone mechanical properties in growing barrows. J Anim Sci 79: 3120-3127
  6. Choi MK, Kim MH. 2004. The analysis of boron contents in 300 Korean common foods. Kor J Community Nutr 9: 837
  7. Naghii MR, Samman S. 1993. The role of boron in nutrition and metabolism. Prog Food Nutr Sci 17: 331-349
  8. Hunt CD, Nielsen FH. 1987. Interactions among dietary boron, magnesium and cholecalciferol in the chick. Proc ND Acad Sci 41: 50
  9. Nielsen FH, Mullen LM, Gallagher SK. 1990. Effect of boron depletion and repletion on blood indicators of calcium status in humans fed a magnesium-low diet. J Trace Elem Exp Med 3: 45-54
  10. Nielsen FH. 1996. Evidence for the nutritional essentiality of boron. J Trace Elem Exp Med 9: 215-229 https://doi.org/10.1002/(SICI)1520-670X(1996)9:4<215::AID-JTRA7>3.0.CO;2-P
  11. Nielsen FH, Hunt CD, Mullen LM, Hunt JR. 1987. Effect of dietary boron on mineral, estrogen, and testosterone metabolism in postmenopausal women. FASEB J 1: 394-397 https://doi.org/10.1096/fasebj.1.5.3678698
  12. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J lmmunol Methods 65: 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  13. Lanoue L, Taubeneck MW, Muniz J, Hanna LA, Strong PL, Murray FJ, Nielsen FH, Hunt CD, Keen CL. 1998. Assessing the effects of low boron diets on embryonic and fetal development in rodents using in vitro and in vivo model systems. Biol Trace Elem Res 66: 271-298 https://doi.org/10.1007/BF02783143
  14. Ding AH, Nathan CF, Stuer DJ. 1988. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J lmmunol 141: 2407-2412
  15. Choi MK, Kim MH, Kang MH. 2005. The effect of boron supplementation on bone strength in ovariectomized rats fed with diets containing different calcium levels. Food Sci Biotehnol 14: 242-248
  16. Weinrab M, Shinar D, Rodan GA. 1991. Different pattern of alkaline phosphatase, ostopontin, and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res 15: 831-842
  17. Palmer RM, Ferrige AG, Moncada S. 1987. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature 327: 524-526 https://doi.org/10.1038/327524a0
  18. Moncada S, Higgs A. 1993. The L-arginine nitric oxide pathway. N Engl J Med 329: 2002-2012 https://doi.org/10.1056/NEJM199312303292706
  19. Yang WW, Krukoff TL. 2000. Nitric oxide regulates body temperature, neuronal activation and interleukin-1$\beta$ gene expression in the hypothalamic paraventricular nucleus in response to immune stress. Neuropharmacology 39: 2075-2089 https://doi.org/10.1016/S0028-3908(00)00054-X
  20. Wise PM. 2002. Estrogens and neuroprotection. Trends Endoerinol Metab 13: 229-230 https://doi.org/10.1016/S1043-2760(02)00611-2
  21. Xia Y, Krukoff TL. 2004. Estrogen induces nitric oxide production via activation of constitutive nitric oxide synthases in human neuroblastoma cells. Endocrinology 145: 4550-4557 https://doi.org/10.1210/en.2004-0327