Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Enhancement of Antigen Presentation Capability of Dendritic Cells and Activation of Macrophages by the Components of Bifidobacterium pseudocatenulatum SPM 1204

  • HAN Shinha (Department of Biology, Seoul Women's University) ;
  • CHO Kyunghae (Department of Biology, Seoul Women's University) ;
  • LEE Chong-Kil (College of Pharmacy, Chungbuk National University) ;
  • SONG Youngcheon (Department of Pharmacy, Sahmyook University) ;
  • PARK So Hee (Department of Pharmacy, Sahmyook University) ;
  • HA Nam-Joo (Department of Pharmacy, Sahmyook University) ;
  • KIM Kyungjae (Department of Pharmacy, Sahmyook University)
  • Published : 2005.09.01
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Abstract

Antigen presenting cells (APCs), dendritic cells (DCs) and macrophages, playa critical role not only in the initiation of immune responses, but also in the induction of immune tolerance. In an effort to regulate immune responses through the modulation of APC function, we searched for and characterized APC function modulators from natural products. Bifidobacterium pseudocatenulatum SPM1204 (SPM1204) isolated from feces of healthy Korean in the age of 20s was used in this experiment. DCs and macrophages were cultured in the presence of supernatants of SPM 1204 and then examined for their activities for the presentation exogenous antigen in association with major histocompatibility complexes (MHC) and macrophage activation. SPM1204 increased class I MHC-restricted presentation of exogenous antigen (cross-presentation) in a DC cell line, DC2.4 cells. The RAW 264.7 cell line was used to test the nonspecific effect of immune reinforcement of SPM1204 as a source of biological regulating modulator for the macrophage activation, include nitric oxide (NO) production and cytokine production. Results showed that the production of NO, tumor necrosis factor (TNF)-$\alpha$, interleukin 1 (IL-1)-$\beta$ and morphological changes in macrophages were largely affected by SPM1204 in a dose-dependent manner. Our results demonstrated that SPM1204 promote cross-presentation of dendritic cells as well as the induction of NO, TNF-$\alpha$ production, and activation of macrophage.

Keywords

References

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