Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Ischemic Preconditioning Ameliorates Hepatic Injury from Cold Ischemia/Reperfusion

  • PARK Sang-Won (College of Pharmacy, Sungkyunkwan University) ;
  • LEE Sun-Mee (College of Pharmacy, Sungkyunkwan University)
  • Published : 2005.09.01
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Abstract

We investigated whether ischemic preconditioning (IPC) protects liver against cold ischemic injury using isolated perfused rat liver. Rat livers were preconditioned by 5 minutes of ischemia and 5 minutes of reperfusion and preserved for 30 hours at $4^{\circ}C$ in University of Wisconsin solution. Livers were then reperfused for 120 minutes. Oxygen uptake and bile flow in ischemic livers markedly decreased during reperfusion. These decreases were prevented by IPC. Portal pressure was elevated in cold ischemic and reperfused livers and this elevation was prevented by IPC. Lactate dehydrogenase and purine nucleoside phosphorylase activities markedly increased during reperfusion. These increases were prevented by IPC. The ratio of reduced glutathione to glutathione disulfide was lower in ischemic livers. This decrease was prevented by IPe. Our findings suggest that IPC protects the liver against the deleterious effect of cold ischemia/reperfusion, and this protection is associated with the reduced oxidative stress.

Keywords

References

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