Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effects of Gonadotropin-Releasing Hormone Analogue (GnRHa) on Expression of the Gonadotropin Subunit Gene and on Synthesis of the Sex Steroids in Black porgy, Acanthopagrus schlegeli

감성돔, Acanthopagrus schlegeli의 GTH$\beta$ 유전자 발현 및 성스테로이드 합성에 미치는 GnRHa의 효과

  • Choi Cheol Young (Division Marine Environment & Bioscience, Korea Maritime University) ;
  • Min Byung Hwa (Department of Aquaculture, Pukyong National University) ;
  • Chang Young Jin (Department of Aquaculture, Pukyong National University) ;
  • Park In-Seok (Division Marine Environment & Bioscience, Korea Maritime University) ;
  • Cho Sung Hwoan (Division Marine Environment & Bioscience, Korea Maritime University) ;
  • An Kwang Wook (Division Marine Environment & Bioscience, Korea Maritime University)
  • 최철명 (한국해양대학교 해양환경 생명과학부) ;
  • 민병화 (부경대학교 수산과학대학 양식학과) ;
  • 장영진 (부경대학교 수산과학대학 양식학과) ;
  • 박인석 (한국해양대학교 해양환경 생명과학부) ;
  • 조성환 (한국해양대학교 해양환경 생명과학부) ;
  • 안광욱 (한국해양대학교 해양환경 생명과학부)
  • Published : 2005.11.01
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Abstract

We examined the effects of GnRHa on expression of the gonadotropin subunit gene in the pituitary and on syn-thesis of the plasma sex steroids (testosterone and 17$\beta$-estradiol) in protandrous black porgy. Fish were injected intraperitoneally with 0.2g GnRHa/g and then both the pituitary and the plasma were sampled 0, 6, 12, 24 and 48 hours after injection. The mRNA level of the FSH subunit increased at 6 hours post-injection, while the LH mRNA levels expressed are same with or without GnRHa treatment. Also, GnRHa stimulation caused a significant increase of the plasma testosterone (T) and 17$\beta$-estradiol ($E_2$) after 24 hours. The homologies of black porgy FSH to red seabream, Pagrus majoy FSH, snakehead fish, Channa maculata FSH and striped bass, Morone saxatilis FSH were $83.3\%,\;79.2\%$ and $76.0\%$ respectively. Amino acid homology analysis using the GenBank and EMBL general searches indicated that black porgy FSH has a high homology with yellowfin seabream, Acanthopagrus latus LH ($97.7\%$ identity) and red seabream LH ($83.3\%$ identity).

RT-PCR을 이용하여 감성돔의 뇌하수체에서 발현하는 FSH$\beta$와 LH$\beta$ cDNA를 각각 cloning하였다. GenBank를 이용하여 FSH$\beta$와 LH$\beta$ 아미노산 배열과 상동성을 비교한 결과, 감성돔 FSH$\beta$는 참돔 FSH$\beta$와 $83.3\%$, snakehead fish FSH$\beta$와 $79.2\%$ 및 striped bass FSH$\beta$와 $76.0\%$의 상동성이 나타났으며 , 감성돔 LH$\beta$는 yellowfin seabream LH$\beta$와 $97.7\%$, 참돔 LH$\beta$와 $96.2\%$의 높은 상동성을 보였다. 또한, 본 연구에서는 1년생 수컷 감성돔에 GnRHa를 처리한 후, 뇌하수체에서 발현하는 GTH subunit mRNA의 농도와 혈중 T및 혈중 $E_2$ 농도의 변화를 조사하였다. GnRHa를 체중 g당 $0.2\;{\mu}g$으로 복강 주사한 후 0, 6, 12, 24 및 48시간제에 뇌하수체와 혈액을 샘플링한 결과, 호르몬 처리 후 6시간째에 FSH$\beta$ mRNA가 유의적으로 증가하였으나, LH$\beta$ mRNA 발현에는 유의적인 차이가 없었다. 또한 혈중 T와 혈중 $E_2$ 농도는 GnRHa처리 후 24시간째 유의적으로 증가하였다.

Keywords

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