Anti-proliferating Effects of Porphyra tenera Fractions on Several Cancer Cell Lines in uitro

김 분획물의 in vitro에서의 항발암효과

This study was performed to investigate the effects of Porphyra tenera (PT) on cytotoxicity and quinone reductase (QR) activity in the cancer cells. PT was extracted with methanol and further fractionated into five different types: hexane (PTMH), ethyl-ether (PTMEE), ethylacetate (PTMEA) butanol (PTMB) and aquous (PTMA) partition layers. We determined the cytotoxic effect of these layers on C6, HepG2, MCF-7, and HT-29 cell lines by MTT assay. Among the various fractions, hexane (PTMH) of PT showed the strongest cytotoxic effect on C6, HepG2 and MCF-7 cell lines. PTMH displayed very low level of cytotoxicity at the lower concentration levels and at 300 $\mu$g/mL. PTMH resulted in 87.5$\%$ growth inhibition on C6 cell 70 $\%$ on the HepG2 cell and 89$\%$ on the MCF-7 cell, which were significantly high compared to other fractions. A 400 $\mu$g/mL PTMH concentration level, 99$\%$, 94.5$\%$ and 99$\%$ of cell growth inhibition were resulted on the same cell lines. On HT-29 cell line, both hexane (PTMH) and aqueous (PTMA) fraction of PT showed cytotoxic effects, but the Percentage was not as high as previous results tested on other cell lines such as C6 HepG2 and MCF-7 cell lines. Also, we observed quinone reductase (QR) inducing-effects in all fractions of PT on HepG2 cells. The QR inducing effects of the PTMH on HepG2 cells at 150 $\mu$g/mL concentration was 6.6 times higher than the control. Although further studies are needed, the present work suggests that PT was a potential to be used as a chemopreventive.

본 실험은 홍조해조류의 하나인 김을 메탄올로 추출 후 각 용매별로 다시 분획하여 암세포 성장억제 효과와 QR유도활성 효과 등 생리활성을 연구하였다. 김을 이용하여 4종의 암세포주 C6, HepG2, MCF-7및 HT-29 세포주에 대한 암세포 증식억제 실험을 한 결과 사용한 4종의 암세포주에서 모두 시료첨가 농도에 의존적으로 증식저지 효과가 나타났고, 특히 김의 hexan 분획물에서 가장 높은 암세포 성장 억제효과를 나타내었다. 그리고 본 시료는 신경종양, 간암 및 여성암의 대표적인 유방암세포의 성장억제효과가 탁월하였다. 또한, 사용한 4가지 암세포주중 유일하게 quinone reductase를 가지고 있는 HepG2를 이용한 암 예방지표인 quinone reductase 효소 유도 활성 여부를 측정한 결과 분획물 첨가농도를 50, 100 및 150 g/mL로 첨가하였을 때 PTMH의 첨가농도 50 ${\mu}g/mL$에서 대조군에 비해 약 1.5배 이상의 높은 QR 유도효과를 나타내었고 최종농도 150 ${\mu}g/mL$에서는 약 6.6배의 높은 암 예방 QR 유도효과를 나타내었다.