Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Investigation of Porcine Melanocortin-4 Receptor (MC4R) Polymorphism on Economic Traits

돼지 melanocortin-4 receptor (MC4R) 유전자의 경제형질과의 연관성에 관한 연구

  • Kim Kwan-Suk (Department of Animal Science, Chungbuk National University) ;
  • Shin Hee Young (Department of Animal Science, Chungbuk National University) ;
  • Lee Joong-Jae (Department of Animal Science, Chungbuk National University) ;
  • Hong Sung-Kwang (Performance Testing Station, Korea Swine Association) ;
  • Choi Bong-Hwan (Animal genomics and Bioinformatics Division, National Livestock Research Institute) ;
  • Kim Tae-Hun (Animal genomics and Bioinformatics Division, National Livestock Research Institute) ;
  • Lee Hak-Kyo (Genetic Information Research Center, Hankyung University) ;
  • Cho Byung-Wook (Department of Animal Science, Miryang National University)
  • Published : 2005.12.01
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Abstract

The Melanocortin-4 receptor gene (MC4R) regulates the energy balance and the genetic basis of obesity. A polymorphism in the porcine melanocortin-4 receptor has previously shown to be associated with growth, fat deposition and feed intake. In this study, the polymorphism of the gene was studied in several pig breeds of Duroc, Landrace, Berkshire, and Yorkshire. The results showed that the frequencies of MC4R genotype varied among those breeds. Association analyses were also performed between the MC4R polymorphism and average daily gain, feed conversion ratio, backfat thickness and lean percentage phenotypes. The results strongly support that the MC4R polymorphism can be used DNA marker selection indicator for economically important traits for pig breeding program in Korea.

본 연구는 Duroc, Landrace, Berkshire, Yorkshire를 기초 축으로 이용한 1003두에 대해 MC4R유전자의 PCR-RFLP를 이용하여 그 다형성을 조사하고 돼지의 일당증체량, 등지방 두께, 사료 요구율, 정육율과 그 유전자형 간의 연관성을 규명하고자 실시하였다. MC4R유전자에 대해 PCR-RFLP를 이용하여 226bp산물을 증폭한후 Taq I 체한효소로 사용하였다. 얻어진 MC4R gene의 유전자 빈도는 품종별로 다르게 나타났다. 통계적 분석을 통하여 각 유전자형에 대한 경제형질과 관련성을 분석한 결과 일당 증체량과 사료요구량은 NN 유전자형을 가진 개체들이 DN이나 DD유전자형을 가진 개체들에 비해 유의적으로 우수한 능력을 보였다(P < 0.05). 하지만 D 대립유전자는 높은 정육율과 낮은 등지방두께에 연관성이 있음을 관찰하였다. 따라서 돼지의 성장과 정육율과 관련된 선발력을 높이기 위해서 MC4R유전자의 다형성분석에서 검증된 PCR marker를 우량돼지육종 계획에 있어 분자생물학적 선발 marker로 사용할 수 있을 것으로 사료된다.

Keywords

References

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