생명과학회지 (Journal of Life Science)

  • 제15권6호
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  • Pages.909-915
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  • 2005
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
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B 세포에서 Epstein-Barr virus microRNA들의 전사 및 성숙

Biosenesis of Epstein-Barr Virus MicroRNAs in B Cells

  • 김도년 (가톨릭대학교 의과대학 생명의과학과 면역생물학연구소) ;
  • 오상택 (가톨릭대학교 의과대학 생명의과학과 면역생물학연구소) ;
  • 이재면 (연세대학교 의과대학 미생물하교실) ;
  • 이원근 (명지대학교 생명과학정보학부) ;
  • 이숙경 (가톨릭대학교 의과대학 생명의과학과 면역생물학연구소)
  • Kim Do Nyun (Research Institute of Immunobiology, Department of Biomedical Sciences, Catholic University of Korea) ;
  • Oh Sang Taek (Research Institute of Immunobiology, Department of Biomedical Sciences, Catholic University of Korea) ;
  • Lee Jae Myun (Department of Microbiology, Yonsei University) ;
  • Lee Won-Keun (Division of Bioscience and Bioinformatics, Myongji University) ;
  • Lee Suk Kyeong (Research Institute of Immunobiology, Department of Biomedical Sciences, Catholic University of Korea)
  • 발행 : 2005.12.01
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초록

우리는 바이러스로는 최초로 miRNA를 생성한다고 보고된 Epstein-Barr virus (EBV)를 대상으로 EBV miRNA biogenesis를 연구했다. 먼저 EVB에 감염된 B 세포인 B95-8로부터 보고된 5 종의 EBV miRNA들인 BHRF1-1, BHRF1-2, BHRF1-3, BART1, 및 BART2 모두가 발현됨을 확인하였다. 그 다음 성숙된 EBV miRNA 서열로부터 예측되는 pri- 와 pre-miRNA들이 B95-8에서 각각 검출되어 EBV miRNA들도 이미 알려진 동물세포 miRNA와 유사한 생성 및 성숙과정을 거칠 가능성을 확인하였다 게놈 상에 2~7개씩 밀집하여 존재하는 동물세포 miRNA들과 유사하게 EBV 게놈의 2부 위에 밀집해서 존재하는 EBV miRNA들도 polycistronic하게 발현되는지 조사한 결과 B95-8에서 BHRF1-1, BHRF1-2 및 BHRF1-3를 포함하는 1,602 뉴클레오타이드의 긴 전사체가 RT-PCR로 확인되었다. 반면 BART1과 BART2는 mono cistronic하게 전사될 가능성이 확인되었다. 본 연구를 통하여 EBV miRNA들도 동물세포 miRNA들과 유사하게 poly cistronic 하게 발현될 수 있으며 pri-와 pre-miRNA 과정을 거쳐 성숙된 miRNA로 생성될 가능성을 확인하였다.

We investigated microRNA (miRNA) biogenesis of Epstein-Barr virus (EBV) which is the first virus shown to produce viral miRNAs. As expected, expression of all the reported EBV miRNAs were detected by Northen blot in an EBV-infected B cell line, B95-8; BHRF1-1, BHIU1-2, BHRF1-3, BART1, and BART2. The putative EBV pri-miRWAs and pre-miRNAs predicted from the known mature EBV miRNA sequences were detected by RT-PCR in B95-8 cells. Many animal miRNA genes exist as clusters of 2-7 genes and they are expressed polycistronically. As the EBV miRNAs are clustered in two regions of the EBV genome, we examined whether these clustered EBV miRNA genes are also expressed polycistronically. A long polycistronic transcript with the expected size (1602 bp) corresponding to the BHRF1-1~BHRF1-2~BHRF1-3 was amplified. However, any polycistronic transcript containing both BART1 and BART2 was detectable in B95-8. These results suggest that EBV miRNAs may be processed in a similar way with animal miRNAs and that some of the clustered EBV miRNAs can be transcribed polycistronically.

키워드

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