생명과학회지 (Journal of Life Science)

  • 제15권6호
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  • Pages.904-908
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  • 2005
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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한국인에서 획득한 Helicobacter pylori의 CagA에 존재하는 SHP-2 binding site의 분석

Analysis of the SHP-2 Binding Site of Helicobacter pylori CagA Protein in Korean

  • 조지윤 (울산대학교 의과대학 서울아산병원 소화기내과) ;
  • 정진용 (울산대학교 의과대학 아산생명과학연구소) ;
  • 강호영 (부산대학교 자연과학대학 미생물학과) ;
  • 김군도 (부경대학교 자연과학대학 미생물학과) ;
  • 변정식 (울산대학교 의과대학 서울아산병원 소화기내과) ;
  • 명승재 (울산대학교 의과대학 서울아산병원 소화기내과) ;
  • 정훈용 (울산대학교 의과대학 서울아산병원 소화기내과) ;
  • 양석균 (울산대학교 의과대학 서울아산병원 소화기내과) ;
  • 홍원선 (울산대학교 의과대학 서울아산병원 소화기내과) ;
  • 김진호 (울산대학교 의과대학 서울아산병원 소화기내과) ;
  • 이진혁 (울산대학교 의과대학 서울아산병원 소화기내과)
  • Jo Ji-Yun (Department of Internal Medicine, Asan Medical Center) ;
  • Jeong Jin-Yong (Asan Institute for Life Scienres, University of Ulsan College of Medicine) ;
  • Kang Ho Young (Department of Microbiology, Pusan National University, Pusan) ;
  • Kim Gun-Do (Department of Microbiology, Pukyong National University) ;
  • Byeon Jeong-Sik (Department of Internal Medicine, Asan Medical Center) ;
  • Myung Seung-Jae (Department of Internal Medicine, Asan Medical Center) ;
  • Jung Hwoon-Yong (Department of Internal Medicine, Asan Medical Center) ;
  • Yang Suk-Kyun (Department of Internal Medicine, Asan Medical Center) ;
  • Hong Weon-Seon (Department of Internal Medicine, Asan Medical Center) ;
  • Kim Jin-Ho (Department of Internal Medicine, Asan Medical Center) ;
  • Lee Gin Hyug (Department of Internal Medicine, Asan Medical Center)
  • 발행 : 2005.12.01
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초록

최근에 발표된 연구 결과에 의하면 pylori CagA내에 존재하는 SHP-2 binding site의 아미노산 서열을 분석한 후, 특정 서열이 위선암의 발병과 연관되어 있다고 보고하였다. 그러나 한국인을 대상으로 CagA 내 에 존재하는 SHP-2 binding site아미노산 서열의 특성을 밝힌 연구는 없다. 따라서 본 연구는 한국인에서 획득한 H. pylori의 CagA SHP-2 binding site에 대해 아미노산 서열을 분석하여 그 특성을 알아보고자 하였다. 총 62 균주의 H. pylori 를 분석한 결과 환자의 질환과 관계없이 모든 H. pylori 균주에서 East Asian (A-B상 혹은 A-B-B-D)을 보여주었다. 일본과 더불어 한국은 위선암 유병률이 높은 나라이므로, 연구 대상의 모든 한국인에서 East Asian type CagA를 가진 H. pylori가 발견된 것이 위선암 유병률과 연관될 가능이 높아 보인다. 그러나 H. pylori의 발병 기전을 보다 더 명확히 이해하기 위해서는 보다 많은 국가와 지역을 대상으로 이러한 유전형 조사가 필요할 것 같다.

Recently the pathological actions of CagA of Helicobacter pylori (H. pylori) on gastric epithelial cells have been reported. CagA is directly injected into the host cytoplasm and undergoes tyrosine phosphorylation in the cells. In addition, translocated CagA forms a physical complex with SHP-2. There are two major CagA subtypes according to the amino acid sequence in the 3'region of CagA; i) the East Asian type (A-B-D of EPIYA motifs) and ii) the Western type (A-B-C of EPIYA motifs). Repeated EPIYA motifs in the 3'region of CagA are involved in the interaction with SHP-2. The East Asian type conferred stronger SHP-2 binding activity than the Westrrn type of CagA. Here we analyzed the amino acid sequences of the SHP-2 binding site of cagA gene in H. pyzori, and investigated whether there is my relationship between the diversities of cagA and the disease out-come in Korea. Most of Korean H. pylori strains showed A-B-D motifs(the East Asian type), and only one strain showed A-B-B-D motifs. In Korea, the incidence of atrophic gastritis and gastric cancer is significantly high compared with Western countries. The high frequency of the East Asian type CagA among Korean H. pylori strains would be involved in increasing the risk of gastric cancer in Korean populations.

키워드

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