Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characterization of a Marine Bacterium Producing Thermotolerant Agarase

내열성 한천분해효소를 생산하는 해양세균의 분리 및 특성

  • Park Ceun-Tae (Research & University-Industry Cooperation, Pusan National University) ;
  • Lee Dong-Ceun (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Kim Nam Young (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee Eo-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jung Jong-Ceun (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Heo Moon-Soo (Paculty of Marine Scienes, Cheju National University) ;
  • Lee Jung-Hyun (Microbiology Laboratory, Korea Ocean Research and Development Institute) ;
  • Kim Sang-Jin (Microbiology Laboratory, Korea Ocean Research and Development Institute) ;
  • Lee Sang-Hyeon (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
  • 박근태 (부산대학교 산학협력단) ;
  • 이동근 (신라대학교 공과대학 생명공학과) ;
  • 김남영 (신라대학교 공과대학 생명공학과) ;
  • 이어진 (신라대학교 공과대학 생명공학과) ;
  • 정종근 (신라대학교 공과대학 생명공학과) ;
  • 이재화 (신라대학교 공과대학 생명공학과) ;
  • 허문수 (제주대학교 해양과학부) ;
  • 이정현 (한국해양연구원 미생물연구실) ;
  • 김상진 (한국해양연구원 미생물연구실) ;
  • 이상현 (신라대학교 공과대학 생명공학과)
  • Published : 2005.12.01
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Abstract

An agar-degrading bacterium was isolated from north-eastern sea of Jeju island and cultured in marine agar 2216 media. Biochemical and morphologicl characteristics and 165 rRNA gene revealed that isolated strain was member of Agarivorans genus, and named Agarivorans sp. JA-1. Agarase was produced as growth-related and expressed regardless of agar presence. Optimal pH was 8 at 50 mM Clycine-NaOH buffer, and activity was maximum at $40^{\circ}C$E Enzymatic activity was maintained over $80\%$ at $60^{\circ}C$t and $70\%$ at $80^{\circ}C$ which is thermotolerant. Hence isolated novel Agarivorans sp. JA-1 strain and its beta-agarase could be used for the production of functional oligosaccharide from agar in solution state.

제주도 동북해역의 해수에서 한천분해활성을 보어는 해양성 세균을 분리하였으며 16S rDNA유전자 염기서열분석과 형태학적, 배양적 및 생화학적 특징을 조사하여 해양기원의 Agarivorans 속에 속하는 균주임을 확인하고 Agarivorans sp. JA-1으로 명명하였다. Agarivorans sp. JA-1이 생성하는 한천 분해효소(agarase)는 한천의 존재유무에 상관없이 발현되며 성장의존성인 것으로 확인되었고 효소활성을 위한 최적 pH는 pH 8.04 (50 mM glycine NaOH 완충용액)이고 최적 온도는 $40^{\circ}C$로 나타났다. 한천분해효소는 기능성올리고당 생산이 가능한 베타-한천분해효소이며 내열성을 보여 $60^{\circ}C$까지 $80\%$ 이상 그리고 $80^{\circ}C$까지 $70\%$의 잔존활성을 보이는 것으로 나타났다. 분리한 Agarivorans sp. JA-1 균주가 나타내는 한천분해효소를 이용하여 $40^{\circ}C$ 이상에서 액체상태로 있는 한천을 이용한 기능성올리고당 생산에 유용한 것으로 생각되었다.

Keywords

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