생명과학회지 (Journal of Life Science)

  • 제15권6호
  • /
  • Pages.863-870
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  • 2005
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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호흡기질환 환자로부터 분리된 Mycoplasma pneumoniae의 tetracycline과 erythromycin에 대한 저항성 변이

Tetracycline and Erythromycin Resistant Mutants of the Mycoplasma pneumoniae Isolated from Patients with Respiratory Diseases

  • 장명웅 (고신대학교 의과대학 미생물학교실) ;
  • 박인달 (고신대학교 의과대학 미생물학교실) ;
  • 김광혁 (고신대학교 의과대학 미생물학교실) ;
  • 송갑영 (부산 위생병원 내과) ;
  • 김성원 (부산 성분도병원 소아과)
  • Chang Myung-Woong (Department of Microbiology, Kosin University College of Medcine) ;
  • Park In-Dal (Department of Microbiology, Kosin University College of Medcine) ;
  • Kim Kwang-Hyuk (Department of Microbiology, Kosin University College of Medcine) ;
  • Song Gap-Young (Department of Internal Medicine, Busan Adventist Hospital) ;
  • Kim Sung-Won (Department of Pediatrics, Busan St. Benedict Hospital)
  • 발행 : 2005.12.01
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초록

2002년 2월부터 2005년 4월까지 호흡기질환 환자로부터 분리된 M. pneumeniae 123 균주의 tetracycline과 erythromycin에 대한 MIC 범위는 각각 $0.5\~1.0$, and $0.5\~512{\mu}/ml$ 이었다. 분리된 M. pneumoniae 123 균주에서 plasmid DNA는 확인되지 않았다. 분리된 M. pneumoniae 123 균주 중에서 57($46.3\%$) 균주가 tetracycline에 저항성인 tetM유전자를 가지고 있었으며, 235 rRNA domain V에 erythromycin에 저항성 변이를 일으킨 균주가 60($48.8\%$)이었다. erythromycin에 저항성 변이를 일으키지 않은 63균주 중에서 tetM 유전자를 가지고 있는 균주는 36($57.1\%$)이었으며, erythromycin에 저항성 변이를 일으킨 60균주 중에서 21($35.0\%$ 균주가 tetM 유전자를 가지고 있었다. 본 연구로써 국내에서 tetracycline과 erythromycin에 대한 저항성 M. pneumoniae 균주의 분리율이 외국에 비하여 높으며, M. pneumoniae 감염의 치료에 erythromycin이 일차 선택제가 될 수 없으므로 이에 대한 범국가적 조사가 필요하다고 생각된다.

One hundred and twenty three strains of Mycoplasma pneumoniae were isolated from patients with respiratory diseases from February 2002 to April 2005 in Busan, Korea. The MICs of tetracycline and erythromycin up to $90\%$ of the 123 M. pneumoniae isolates tested were $0.5\~1.0$, and $0.5\~512{\mu}/ml$, respectively. Plasmid DNA was not isolated from all of the M. pneumoniae isolates. Out of 323 strains of M. pneumoniae, 57 ($46.3\%$) stains contain tetM gene on their chromosomal DNA, and 60 ($48.8\%$) strains were mutated in domain V of 23S rRNA for erythromycin resistance. Out of 63 strains of M. pneumoniae which were not mutated in domain V of 235 rRNA for erythromycin resistance, 36 ($57.1\%$) strains contained tetM gene, and out of 60 strains of M. pneumoniae which were mutated in domain V of 23S rRNA for erythromycin resistance, 21 ($35.0\%$) strains contained tetM gene. These results suggest that the isolation rate of erythromycin and tetracycline resistant M. pneumoniae is higher than those of other countries, and erythromycin and tetracycline are not first choice drug for M. pneumoniae infection in Korea, and it need confirm by a nationwide surveilance of antimicrobial resistance.

키워드

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피인용 문헌

  1. Multi-drug Resistance of Mycoplasma pneumoniae Isolates from Patients with Respiratory Diseases against Quinolone and Macrolide vol.17, pp.3, 2007, https://doi.org/10.5352/JLS.2007.17.3.435
  2. Detection of genetic mutations associated with macrolide resistance of Mycoplasma pneumoniae vol.53, pp.2, 2010, https://doi.org/10.3345/kjp.2010.53.2.178