생명과학회지 (Journal of Life Science)

  • 제15권6호
  • /
  • Pages.847-850
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  • 2005
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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형질 전환된 Saccharomyces cerevisiae의 leucocin A항균 활성도에 대한 탄소원의 영향

Effects of Carbon Source on Production of Leucocin A from Transformed Saccharomyces cerevisiae

  • 이성일 (신라대학교 공과대학 생명공학과) ;
  • 박진용 (신라대학교 공과대학 생명공학과) ;
  • 정종근 (신라대학교 공과대학 생명공학과) ;
  • 이동근 (신라대학교 공과대학 생명공학과) ;
  • 이상현 (신라대학교 공과대학 생명공학과) ;
  • 하종명 (신라대학교 공과대학 생명공학과) ;
  • 하배진 (신라대학교 공과대학 생명공학과) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • Lee Sung-ll (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Park Jin-Yong (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jung Jong-Ceun (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee Dong-Ceun (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee Sang-Hyeon (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Ha long-Myung (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Ha Bae-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
  • 발행 : 2005.12.01
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초록

B. subtilis에 대한 항균 활성을 파악하기 위하여 bacteriocin의 일종인 leucocin A로 형질전환된 효모를 배양 시간별로 채취하여 광학밀도, 총 분비 단백질량, 단백질 분해효소 그리고 향균활성을 측정했다. Sucrose > glycerol > glucose > fructose 순으로 세포 증식이 높게 확인되었고, glyceroldmf 제외한 탄소원에서 총분비 단백질은 배양 15 시간까지 증가되었다. Leucocin A의 향균활성(antibacterial activity)은 성장양상, 총 분비 단백질과 비례 하였으며 배양 15 시간에 glucose와 sucrose에서 배양한 상등액이 B. subtilis 성장을 가각 $65.5\%$, $72.6\%$ 감소하는 것으로 나타났다. 정지기 이후에는 향균활성이 급속히 감소하였는데, 이는 항균활성을 보이는 leucocin A가 단백질이고, 단백질 분해효소가 정지기 이후 증가한 것에 의한 것으로 사료된다. 본 연구는 향후 식품산업뿐만 아니라 항생제 생산 사용되기 위한 산업적 기초 자료로써 이용 될 것이다.

The aim of this study was to increase production of leucocin A, a kind of bacteriocin, in a transformed variety of S. cerevisiae. We investigated optical density, total secreted protein, protease activity, and antibacterial activity for the transformed S. cerevisiae in different carbon sources. The production of leucocin A growth-associated, and antibacterial activity, according to carbon source, was in the order of sucrose, glucose, glycerol, and fructose. Antibacterial activity was $10.6\%$ higher in the presence of sucrose than glucose. This is the first report regarding the effect of carbon sources on the production of leucocin A in transformed S. cerevisiae, as far as we ascertain. Our results could prove useful in the industrial production of natural preservatives.

키워드

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