Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Influence of Bakers' Yeast Cells on Protein Adsorption Performance in Dye-Ligand Expanded Bed Chromatography

  • Chow, Yen Mei (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Tey, Beng Ti (Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Ibrahim, Mohd Nordin (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Ariff, Arbakariya (Department of Bioprocess Technology, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia) ;
  • Ling, Tau Chuan (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)
  • Published : 2005.12.31
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Abstract

The influence of whole yeast cells $(0{\sim}15%\;w/v)$ on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent $(p=1.5\;g/cm^3)$ derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a $C/C_0=0.1$ biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5mg/mL of settled adsorbent, respectively.

Keywords

References

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