Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidant Activity on Ethanol Extract from Boiled-water of Hizikia fusiformis

톳 자숙액 에탄올 추출물의 항산화 활성

  • Park, Ki-Eui (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Jang, Mi-Soon (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Lim, Chi-Won (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Kim, Yeon-Kye (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Seo, Young-Wan (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Park, Hee-Yeon (Biotechnology Research Center, National Fisheries Research & Development Institute)
  • 박기의 (국립수산과학원 생명공학연구단) ;
  • 장미순 (국립수산과학원 생명공학연구단) ;
  • 임치원 (국립수산과학원 생명공학연구단) ;
  • 김연계 (국립수산과학원 생명공학연구단) ;
  • 서영완 (한국해양대학교 해양환경.생명과학부) ;
  • 박희연 (국립수산과학원 생명공학연구단)
  • Published : 2005.12.31
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Abstract

Antioxidant activity of the ethanol extract from boiled-water of Hizikia fusiformis (EBH) were compared with those of BHA, L-ascorbic acid, gallic acid, caffeic acid and (-)-catechin. The free radical scavenging ability against DPPH (1,1-diphenyl-2-picrylhydrazyl), authentic peroxynitrite and reducing power were measured as indices of antioxidant activity. EBH showed the potent DPPH radical and peroxynitrite scavenging activities, showing 85.23 and 96.97% at final concentration of $1000{\mu}g/ml$, respectively. The reducing power increased with the increasing amount of EBH (final concentration of 1, 10, 100 and $1000{\mu}g/ml$). Total phenolic content of EBH was 588 mg (-)-catechin/g at the final concentration $1000{\mu}g/ml$. Total phenolic contents correlated with DPPH radical scavenging activity $(R^2=0.766)$ and reducing power $(R^2=0.944)$. These results suggested that EBH could be a natural antioxidative source containing antioxidative components.

Keywords

References

  1. Suzuki, T., Nakai, K., Yoshie, Y., Shirai, T. and Hirano, T. (1993) Effect of sodium alginates rich in guluronic acid and mannuronic acids on cholesterol levels and digestive organs of high-cholesterol-fed rats. Nippon Suisan Gakkaishi, 59, 545-551
  2. Kim, S. H., Lim, S. B., Ko, Y. H., Oh, C. K., Oh, M. C. and Park, C. S. (1994) Extraction yields of Hizikia fusiforme by solvents and their antimicrobial effects. Bull. Korean Fish. Soc. 27, 462-468
  3. Bae, S. J. (2004) Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J. Korean Soc. Food Sci. Nutr. 33, 480-486
  4. Kim, S. A., Kim, J., Woo, M. K., Kwak, C. S. and Lee, M. S. (2005) Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J. Korean Soc. Food Sci. Nutr. 34, 451-459 https://doi.org/10.3746/jkfn.2005.34.4.451
  5. Lee, B. H., Choi, B. W., Chun, J. H. and Yu, B. S. (1996) Extraction of water soluble antioxidants from seaweeds. J. of Korean Ind. & Eng. Chemistry 7, 1069-1077
  6. Ko, M. S., Shin, K. M. and Lee, M. Y. (2002) Effects of Hizikia fusiforme ethanol extract on antioxidative enzymes in ethanol-induced hepatotoxicity of rat liver. J. Korean Soc. Food Sci. Nutr. 31, 87-91
  7. Arasaki, S. and Arasaki, T. (1983) In vegetables from the sea. Japan Publications Inc., Tokyo
  8. Kim, K. I., Seo, H. D., Lee, H. S., Jo, H. Y. and Yang, H. C. (1998) Studies on the blood anticoagulant polysaccharide isolated from hot water extracts on Hizikia fusiforme. J. Korean Soc. Food Sci. Nutr. 27, 1204-1210
  9. Ryu, B. H., Kim, D. S., Cho, K. and Sin, D. B. (1989) Antitumor activity of seaweeds agarne sarcoma-180. Korean J. Food Sci. Technol. 21, 595-600
  10. Kim, S. H. (1990) Antitumor effect of water-soluble extracts of plants-herbs, seaweeds, and mushrooms-in Cheju island. M. S. Thesis, Cheju National University, Cheju
  11. Yan. X., Yoshihiro, C., Masahiro, S. and Tadahiro N. (1999) Fucoxanthin as the major antioxidant in Hizikia fusiformis, a common edible seaweed. Biosci. Biotechnol. Biochem. 63, 605-607 https://doi.org/10.1271/bbb.63.605
  12. Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 26, 1158
  13. Kooy, N. W., Royall, J. A., Ischiropoulos, H. and Beckman, J. S. (1994) Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free radical Biology & Medicine. 16, 149-156 https://doi.org/10.1016/0891-5849(94)90138-4
  14. Oyaizu, M. (1986) Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44, 307-315
  15. Cheung, L. M., Cheung, P. C. K. and Ooi, V. E. C. (2003) Antioxidant activity and total phenolic of edible mushroom extracts. Food Chem. 81, 249-255 https://doi.org/10.1016/S0308-8146(02)00419-3
  16. Kaneda, T. and Ando, H. (1971) Component lipids of purple laver and their antioxigenic activity. Proc. Int. Seaweed Symp. 7, 553
  17. Fujimoto, K. and Kaneda, T. (1984) Separation of antioxidant compounds from marine algae. Hydrobiologia, 116
  18. Pryor, W. and Squadrito, G. (1995) The chemistry of peroxynitrite: a product from the reaction of nitric oxide with the superoxide anion. A. J. Physiol. 264, 699-722
  19. Reiter, R. J., Tan, D. X. and Burkhardt, S. (2002) Reactive oxygen and nitrogen species and cellular and organismal decline: amelioration with melatonin. Meeh. Aging. Dev. 123, 1007-1019 https://doi.org/10.1016/S0047-6374(01)00384-0
  20. Virag, L., E. Szabo, P. Gergely and C. Szabo (2003) Peroxynitrite induced cytotoxicity: mechanism and opportunities for intervention. Toxicol. Lett. 140-141, 113-124
  21. Siddhuraju, P., Mohan, P. S. and Becker, K. (2002) Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chem. 79, 61-67 https://doi.org/10.1016/S0308-8146(02)00179-6
  22. Meir, S., Kanner, J., Akiri, B. and Hadas, S. P. (1995) Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agric. Food Chem. 43, 1813-1815 https://doi.org/10.1021/jf00055a012
  23. Gulcin, I., Buyukokuroglu, M. E., Oktay, M. and Kufrevioglu, O. I. (2003) Antioxidant and analgesic activities of turpentine of Pinus nigra Am. subsp. pallsiana (Lamb) Holmboe. J. Ethnopharmaeol. 86, 51-58 https://doi.org/10.1016/S0378-8741(03)00036-9
  24. Lu, Y. and Foo, L. Y. (2000) Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68, 81-85 https://doi.org/10.1016/S0308-8146(99)00167-3
  25. Kim, Y. C. and Chung, S. K. (2002) Reactive oxygen radical species scavenging effects of Korean medicinal plant leaves. Food Sci. Biotechnol. 11, 407-411
  26. Pyo, Y. H., Lee, T. C., Logendra, L. and Rogen, R. T. (2004) Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chem. 85, 19-26 https://doi.org/10.1016/S0308-8146(03)00294-2
  27. Park, Y. K., Lee, W. Y., Park, S. Y., Ahn, J. K. and Han M. S. (2005) Antioxidant activity and total phenolic content of Callistemon citrinus extracts. Food Sci. Biotechnol. 14, 212-215