Archives of Pharmacal Research

  • 제28권10호
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  • Pages.1170-1176
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  • 2005
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Suppressive Effects of Furonaphthoquinone NFD-37 on the Production of Lipopolysaccharide-Inducible Inflammatory Mediators in Macrophages RAW 264.7

  • Kim Min-Hee (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Shin Hyun-Mo (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Yong Rok (School of Chemical Engineering and Technology, Yeungnam University) ;
  • Chung Eun Yong (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Chang Yoon Sook (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Min Kyung Rak (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim Youngsoo (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University)
  • 발행 : 2005.10.01
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초록

2-Methyl-2-(2-methylpropenyl)-2,3-dihydronaphthoquinone[2,3-b]furan-4,9-dione (N FD-37) is a synthetic furonaphthoquinone compound. In this study, we determined that NFD-37 could inhibit the lipopolysaccharide (LPS)-induced production of inflammatory mediators in macrophages RAW 264.7. This compound inhibited LPS-induced nitric oxide (NO) or prostaglandin (PG) $E_{2}$ production in dose-dependent manners, with $IC_{50}$ values of 7.2 ${\mu}M$ and 5.3 ${\mu}m$, respectively. As the positive controls, pyrrolidine dithiocarbamate (30 ${\mu}M$) exhibited a $57{\%}$ inhibition of NO production, and NS-398 ($1{\mu}M$) manifested a $48{\%}$ inhibition of $PGE_2$ production. The inhibitory effects of NFD-37 on NO and $PGE_2$ production were determined to occur in conjunction with the suppression of inducible NO synthase or cyclooxygenase-2 expression. NFD-37 also inhibited the production of LPS-inducible tumor necrosis factor-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-6, at $IC_{50}$ values of 4.8-8.9 ${\mu}M$. We also determined the anti-inflammatory efficacy of NFD-37 using carrageenin-induced paw edema in experimental mice.

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