Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of Folio Acid Supplementation on Plasma Homocysteine and Thiobarbituric Acid Reactive Substances (TBARS) Levels and Liver SAM/SAH Ratio in Hyperhomocysteinaemia-induced Pregnant Rats

고호모시스테인혈증 임신 흰쥐에서 엽산보충이 혈장 호모시스테인, Thiobarbituric Acid Reactive Substances (TBARS) 수준과 간의 SAM/SAH에 미치는 영향

  • Hong, Kyounk-Ju (Department of Food and Nutritional of Science, Asia Food & Nutrition Research Institute Ewha Womans University) ;
  • Hyun, Tai-Sun (Department of Food and Nutrition, Chnugbuk National University) ;
  • Chank, Nam-Soo (Department of Food and Nutritional of Science, Asia Food & Nutrition Research Institute Ewha Womans University)
  • 홍경주 (이화여자대학교 생활환경대학 식품영양학과) ;
  • 현태선 (충북대학교 생활과학대학 식품영양학과) ;
  • 장남수 (이화여자대학교 생활환경 대학 식품영양학과)
  • Published : 2005.09.01
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Abstract

This study was performed to investigate effects of dietary folic acid supplementation on plasma homocysteine levels, thiobarbituric acid reactive substance s (TBARS) level s and liver SAM/SAH ratio in hyperhomocysteinaemia-induced pregnant rats. Forty-two female Sprague-Dawley rats were divided three groups (C: control diet, HFD: $0.3\%$ homocystine and 0 mg folic acid diet, HFS: $0.3\%$ homocystine and 8 mg/kg folic acid diet) according to homocystine and folic acid levels in the diet. They were fed experimental diets for 5 weeks prior to the mating and also during the entire period of pregnancy till gestational day 20. Dietary folic acid supplementation caused a significant decrease in plasma homocysteine levels which had been increased by a homocystine-diet, with a concomitant increase in plasma and liver folate levels. Liver TBARS levels in homocysteine-folic acid-deficient group (HFD) were higher than those in control group. Dietary folic acid supplementation increased hepatic SAM/SAM ratio in homocysteine-folic acid- sopplemetantion group (HFS) when compared to the HFD (p < 0.05). These data suggest that folate depletion and elevated plasma homocysteine may promote oxidative stress in rat livers and influence the remethylation cycle of the homocysteine metabolism detrimentally. In conclusion, dietary folic acid supplementation was found to be effective for lowering plasma homocysteine levels, relieving oxidative stress, and improving the methylation status in the body.

Keywords

References

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