Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Hypoglycemic and Antioxidative Effects of Fermented Chaga Mushroom (Inonotus obliquus) on Streptozotocin-induced Diabetic Rats

Streptozotocin 유발 당뇨쥐에서 발효 차가버섯의 항당뇨 및 항산화 효과

  • Cha Jae-Young (Department of Biotechnology, College of Natural Resources and Life Science,Dong-A University) ;
  • Jun Bang-Sil (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Lee Chi-Hyeoung (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Yooi Ki-Soo (Department of Anatomy, College of Medicine, Dong-A University) ;
  • Moon Jae-Chul (Hong Jae Green Co., Ltd) ;
  • Cho Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • 차재영 (동아대학교 응용생명공학부) ;
  • 전방실 (동아대학교 응용생명공학부) ;
  • 이치형 (동아대학교 응용생명공학부) ;
  • 유기수 (동아대학교 의과대학 해부학교실) ;
  • 문재철 (홍재그린(주)) ;
  • 조영수 (동아대학교 응용생명공학부)
  • Published : 2005.10.01
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Abstract

The effects of fermented chaga mushroom (Inonotus obliquus) on the concentrations of serum glucose, insulin, lipids and lipid peroxidation in streptozotocin (STZ)-induced diabetic rats were investigated. Rats were fed a semisynthetic diet supplemented with 50 g/kg chaga mushroom powder (the CM group) and fermented chaga mushroom powder (the FCM group), and no supplemented (the control group) for 3 weeks. The polysaccharide concentrations were CM by $42.9\%$ and FCM by $ 39.1\%$, and the total polyphenol concentrations were CM by $ 0.80\%$ and FCM by $0.91\%$. Feed intakes and water consumption, serum glucose, insulin, triglyceride, and blood urea nitrogen concentrations were significantly lower in the FCM group than in both the CM and control groups. The activities of AST and ALT were also significantly lower in the FCM group than in the control group. No significant differences were detected with regard to the serum cholesterol and creatinine concentrations among the experimental groups. Lipid peroxidations in hepatic homogenate, microsomal and mitochondrial subcellular and pancreas were significantly lowered by the administration of FCM in the STZ-diabetic rats. Hepatic glutathione concentrations, which is closely associated with antioxidant system, was significantly higher in the FCM group than in the control group, indicating a marked effect of FCM administration on the endogenous antioxidant system. However, CM treatment showed a moderate antioxidative activity in the STZ-diabetic rats. Our results indicate that fermented chaga mushroom exert hypoglycemic and antioxidative effects in type 1 diabetes mellitus.

차가버섯 (Inonotus obliquus) 분말을 Bacillus sp.로 발효시켜 streptozotocin (STZ)-유발 당뇨쥐에 $ 5\%(w/w)$ 수준으로 첨가한 반합성 식이를 3주간 투여하여 혈당치 및 과산화지질 농도에 미치는 영향을 검토하였다. 차가버섯 및 발효 차가버섯의 다당체는 각각 $42.9\%$ 및 $39.1\%$였으며, 폴리페놀 화합물 함량은 각각 $0.80\%$및$0.91\%$였다. 식이 및 음료 섭취량, 혈청 중의 glucose, insulin, triglyceride, blood urea nitrogen농도는 당뇨 대조군과 차가버섯 투여군 보다는 발효 차가버섯 투여군에서 현저히 감소하였다. 혈청 중의 간 기능 임상치료 효소인 AST 및 ALT 활성도 발효 차가버섯 투여군에서 역시 감소하였다. 그러나 혈청 total cholesterol 및 creatinine 농도는 각 실험군간에 유의적인 차이는 없었다. 한편, 간 조직의 homogenate, microsomal 및 mitochondria 분획의 과산지질 농도는 당뇨쥐에 발효 차가버섯 투여로 현저히 감소하였다. 간 조직에서 내인성 항산화물질로 알려져 있는 glutathione 농도가 발효 차가버섯 투여군에서 현저히 증가함으로써 과산화지질 농도 억제에 의한 항산화 활성과 밀접한 관련성을 가진 것으로 나타났다 차가버섯 투여군에서는 발효 차가버섯 보다는 미약한 항산화 활성을 보였다. 이상의 결과에서 발효 차가버섯은 streptozotocin-유발 당뇨쥐에서 우수한 항당뇨 및 항산화 효과를 발휘하였다.

Keywords

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