Characterization of Antioxidants Extracted from Leaves of Sanjook(Sasa boreails var. chiisanensis)

산죽잎으로 부터 추출한 항산화물질의 특성

For usage of natural antioxidants, sanjook (Sasa boreails var. chiisanensis) leaves were extracted with methanol and investigated about its antioxidative activities and stability. It showed that the antioxidant activity of methanol extracts from the leaves of sanjook depend on their concentration within range of 0.1 to 0.8 mg/ml. The methanol extracts from the leaves of sanjook represented $583{\mu}g/ml$ for $IC_{50}$ of DPPH radical scavenging ability, $800{\mu}g/ml$for $IC_{50}$ of SOD-like activity and $38{\mu}g/ml$for $IC_{50}$ of $H_{2} O_{2}$ scavenging ability, while BHT, as a compared substance, was $271{\mu}g/ml$ for $IC_{50}$ of DPPH radical scavenging ability, $680{\mu}g/ml$ for $IC_{50}$ of SOD-like activity and $30{\mu}g/ml$ for $IC_{50}$ of $H_{2} O_{2}$ scavenging ability, respectively. The anti-au-toxidation effect for methanol extracts from the leaves of sajook was $55\∼60\%$ within range of 0.1 to 0.8 mg/ml. The pH stability on methanol extracts from the leaves of sanjook was most stable at pH 6. The more acid or akali it became, the more unstable it turned. The thermostability on methanol extracts from the leaves of sanjook remained above $80\%$ of their DPPH activity at range of $0^{\circ}C{\;}to{\;}120^{\circ}$.

국내산 산죽잎을 methanol로 추출하여 천연 항산화제로서의 이용성을 조사하기 위하여 여러 방법으로 항산화 활성을 측정하고 안정성에 대해 조사하였다. 산죽잎 메탄올 추출물은 0.1 mg/l에서 0.8mg/ml의 범위에서 농도 의존적으로 항산화활성이 증가하였다. 산죽잎 메탄올추출물은 DPPH radical 소거능에 대한 $IC_{50}$ 값은 $583{\mu}g/ml$, SOD 유사활성능에 대한 $IC_{50}$값은 $800{\mu}g/ml$, hydrogen peroxide 소거능에 대한 $IC_{50}$값은 $38{\mug}/ml$이 었으며 대조구로 사용한 BHT의 각각 의 $IC_{50}$값은 $271{\mu}g/ml,{\;}680{\mu}g/ml,{\;}30{\mu}g/ml$이었다. 산죽잎 메탄올추출물의 자동산화 억제능은 상기 농도 범위에서 $55\∼60\%$의 억제효과를 나타내었다. 산죽잎 메탄올추출물의 pH 안정성은 pH 6에서 안정하였으나 산성이나 알카리성으로 갈수록 pH 안정성은 떨어졌다. 산죽잎 메탄올추출물의 열안정성은 $0^{\circ}C$에서 $120^{\circ}C$의 온도범위에서 $80\%$ 이상의 항산화능을 유지하였다.