생명과학회지 (Journal of Life Science)

  • 제15권5호
  • /
  • Pages.715-725
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  • 2005
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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자생 및 약용 식물의 Streptococcus mutans항균 활성의 검색

Screening of Antibacterial Agent Against Streptococcus mutans from Natural and Medicinal Plants,

  • 김건우 (안동대학교 생약자원학과) ;
  • 백정규 (안동대학교 생약자원학과) ;
  • 장영욱 (안동대학교 생약자원학과) ;
  • 금은주 (안동대학교 식품영양학과) ;
  • 권윤숙 (안동대학교 식품영양학과) ;
  • 김홍주 (안동대학교 식품영양학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Kim Kun-Woo (The School of Bioresource Sciences, Andong National University) ;
  • Baek Jueng-Kuy (The School of Bioresource Sciences, Andong National University) ;
  • tang Young-Wook (The School of Bioresource Sciences, Andong National University) ;
  • Kum Eun-Joo (Dept. of Food and Nutrition, Andong National University) ;
  • Kwon Yun-Suk (Dept. of Food and Nutrition, Andong National University) ;
  • Kim Hong-Ju (Dept. of Food and Nutrition, Andong National University) ;
  • Sohn Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • 발행 : 2005.10.01
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초록

식용 및 약용 식물 215 종의 천연물로부터 추출부위를 달리한 메탄올 추출물 309 종을 조제하였으며, 안전성이 우수한 천연물 충치예방 및 치료제 개발을 목표로 309종의 추출물의 S. mutans JC-2에 대한 항균 활성을 평가하였다. 항균활성은 96 well microtiter method에 의한 생육저해능 측정 및 disk paper method에 의한 생육저지환 크기를 측정하여 평가하였으며, 그 결과 가죽나무(가지), 곰딸기(열매), 독활(잎), 상수리나무(잎), 여뀌(전초), 작약(종자), 짚신나물(뿌리) 추출물에서 강한 활성을 확인하였다. 상기 7종 천연물의 최소 생육저지농도(MIC)를 측정하여 항균 활성을 비교한 결과, 여뀌, 상수리나무, 가죽나무 추출물이 $25\∼30{\mu}g/ml $의 농도에서 생육을 완전히 억제하였다 이러한 결과는 항균력이 우수한 천연물로부터 새로운 충치 예방 및 치료제 개발이 가능함을 제시한다

Oral streptococci are major constituents of dental plaques, and their prevalence is closely linked with various pathologic symptoms, such as dental caries. To develop natural anticaries agent, we prepared 309 kinds of plant extracts from 215 species of edible or medical plants, and antibacterial activity of the extracts against Streptococcus mutans JC-2 were evaluated based on 96 well microtiter plate assay and disk paper method, subsequently. Among the tested plant extracts, Ailanthus altissima, Paeonia lactiflora, Rubus phoenicolasius, Aralia continentalis, Quercus acutissima, Persicaria hydropiper and Agrimonia pilosa extracts showed strong antimicrobial activity. Determination of minimal inhibitory concentration (MIC) of the selected seven plant extracts showed that Ailanthus altissima, Persicaria hydropiper and Quercus acutissima extracts ($MIC=25\∼30[\mu}g/ml$) has potential as a source of natural anticaries agents.

키워드

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