Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Combined Eeffect of Exercise and L-arginine Supplementation on Cardiovascular and Immune Responses in SHR

L-arginine 투여와 훈련이 SHR의 심혈관 반응과 면역력에 미치는 영향

  • Kawk Yi-Sub (Department of Leisure and Sports Science, Dongeui University)
  • 곽이섭 (동의대학교 레저스포츠학과)
  • Published : 2005.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study is to investigate the combined effect of L-arginine supplementation and regular physical exercise on HR, BP, eNOS and Macrophage activation using SHR. To examine the differences among HR, BP, eNOS, and Macrophage activity levels, normotensive Wistar-Kyoto rats were used as a control. Thirty two male rats (six weeks old) were divided into four groups; eight WKY control (WKYC), eight SHR control (SHRC), eight SHR supplemented with L-arginine (SHRA), and eight SHR trained and supplemented with L-arginine (SHRTA). Obtained results were as follows : In the heart and blood pressure, there was significant differences anong the four group (p<.05) compare to SHRC. In the eNOS levels, there was significant differences among the four groups (p<.05) compare to SHRC. In the macrophage activity, there was significant differences among the four groups (p<.05) compare to SHRC. In conclusion, For the SHRC group, the level of eNOS is higher than that of WKYC, and we can expect tissue damage caused by toxic free radical. However, this can be stabilized by the L-arginine supplementation and regular physical training. we can also conclude regular aerobic training decrease cardiovascular stress caused by stabled macrophage activity. Therefore, we can trace it is the effect of training in SHR.

본 연구는 Wistar-Koyto Rat과 Spontaneously Hypertensive Rat을 대상으로 10주간의 L-arginine 투여와 규칙적인 수영 훈련을 하였을 때, 심박수, 혈압, 간조직의 eNOS 활성 및 대식세포의 활성수준을 비교 분석하여 고혈압 질환상태에서 심혈관계 및 eNOS 수준에 대한 L-arginine 투여와 훈련의 효과를 규명하는데 목적이 있었다. 결과적으로 고혈압 통제군의 심박수, 혈압, eNOS, 및 대식세포의 활성은 정산군에 비해 현저하게 높은 수치를 보였는데, 이는 고혈압으로 인한 혈관내피세포 손상으로 산화질소 생성기전에 불안정을 초래하여 심혈관계에 부정적인 영향을 주었을 것으로 해석한다. 하지만 L-arginine 투여와 규칙적인 수영훈련은 심박수, 혈압 및 eNOS 수준을 안정 수준으로 회복하였고, 대식세포의 활성 역시 안정화함으로 심혈관계에 긍정적인 영향력을 미치는 것으로 나타났다.

Keywords

References

  1. Sainani, G. S. and Maru, V. G. 2004. Role of endothelial cell dysfunction in essential hypertension. J. Assoe. Physicians. India. 52, 966-969
  2. Zanetti, M., d'Uscio, J. V., Peterson, T. E., Katusic, Z. S. and O'brien, T. 2004. Analysis of superoxide anion production in tissue. Methods Mol. Med. 108, 65-72
  3. Moe, I. T., Hoven, H., Hetland, E. V., Rognmo, O. and Slordahl, S. A. 2005. Endothelial function in highly endurancetrained and sedentary, healthy young woman. Vase. Med. 10(2), 97-102 https://doi.org/10.1177/1358836X0501000114
  4. Yamamoto, K., Ikeda, U. and Shimada, K. 2003. Role of mechanical stress in monocytes/macrophages: implications for atherosclerosis. Curro Vase. Pharmacal. 1(3), 315-319 https://doi.org/10.2174/1570161033476565
  5. Choudhury, A. and Lip, G. Y. 2005. Exercise and hypertension. J. Hum. Hypertens. 19(8), 585-587 https://doi.org/10.1038/sj.jhh.1001851
  6. Stewart, K. J., Bacher, A. C., Turner, K. L., Fleg, J. L., Hees, P. S., Shapiro, E. P., Tayback, M. and Ouyang, P. 2005. Effect of exercise on blood pressure in older persons: a randomized controlled trial. Arch. Intern. Med. 11;165(7), 756-762
  7. Hsieh, N. K., Wang, J. Y., Liu, J. C., Wang, S. D. and Chen, H. J. 2004. Nitric oxide inhibition accelerates hypertension and induces perivascular inflammation in rats. Clin. Exp. Pharmacal. Physial. 31(4), 212-218 https://doi.org/10.1111/j.1440-1681.2004.03977.x
  8. Simko, F., Luptak, I., Matuskova, L Krajcirovicova, K., Sumbalova, Z., Kucharska, J., Gvozdjakova, A., Simko, J., Babal, P., Pechanova, O. and Bernatova, J. L-arginine fails to protect against myocardial remodelling in L-NAMEinduced hypertension. Eur. J. Clin. Invest. 35(6), 362-368 https://doi.org/10.1111/j.1365-2362.2005.01507.x
  9. Hwu, C. M., Hsiao, C. F., Kuo, S. W., Wu, K. D., Ting, C. T., Quertermous, T., Rodriguez, B., Chen, I., Grove, J., Chen, P. Y. and Hoo, L. T. 2004. Physical inactivity is an important lifestyle determinant of insulin resistance in hypertensive patients. Blood press. 13(6), 355-361 https://doi.org/10.1080/08037050410004828
  10. Baster, T. and Baster-Brooks, C. 2005. Exercise and hypertension. Aust. Fam. physician. 34(6), 419-424
  11. Scheen, A. J. and Giet, D. 2005. Prevention of type 2 diabetes: a new public health challenge. Rev. Med. Liege. 60(5-6), 383-390
  12. Chang, C. C., Huang, H. C., Wang, S. S., Lee, F. Y., Chang, F. Y., Lin, H. C., Hou, M. C. and Lee, S. D. 2005. Involvement of constitutive nitric oxide synthase in the portal-systemic collaterals of portal hypertensive rats. J. Chin. Med. Assoc. 68(6), 245-249 https://doi.org/10.1016/S1726-4901(09)70144-3
  13. Brennan, P., Pescatello, L. S., Bohannon, R. W., Marschke, L., Murphy, M. Coble, D. and Hasson, S. 2005. Time spent moving is related to systolic blood pressure among older women. Prevo Cardial. 8(3), 160-164 https://doi.org/10.1111/j.1520-037X.2005.3820.x
  14. Llorens, S., Salazar, F. J. and Nava, E. 2005. Assessment of the nitric oxide system in the heart, aorta and kidney of aged Wistar-Kyoto and spontaneously hypertensive rats. J. Hypertens. 23(8), 1507-1514 https://doi.org/10.1097/01.hjh.0000173122.31848.f3
  15. Bolad, J. and Delafontaine, P. 2005. Endothelial dysfunction: its role in hypertensive coronary disease. Curro Opin. Cardial. 20(4), 270-274 https://doi.org/10.1097/01.hco.0000167719.37700.1d