The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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In Vivo Antifungal Activities of Various Fungicides against Plamodiophora brassicae

다양한 살균제의 배추 뿌리혹병 방제효과

  • Jang, Kyoung-Soo (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Lim, He-Kyoung (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Cho, Kwang-Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung-Ja (Biological Function Research Team, Korea Research Institute of Chemical Technology)
  • 장경수 (한국화학연구원 생물기능연구팀) ;
  • 김진철 (한국화학연구원 생물기능연구팀) ;
  • 임희경 (한국화학연구원 생물기능연구팀) ;
  • 조광연 (한국화학연구원 생물기능연구팀) ;
  • 최경자 (한국화학연구원 생물기능연구팀)
  • Published : 2005.12.30
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Abstract

In vivo antifungal activity of 44 fungicides consisting of 3 clubroot fungicides, 7 Oomycetes fungicides, 7 botriticides, 7 blasticides, 9 sterol biosynthesis inhibitors, and 11 broad spectrum fungicides were investigated against Plamodiophora brassicae, the causal agent of clubroot disease in Chinese cabbage. When fluazinam, flusulfamide and cyazofamid, commercial fungicide to control clubroot of Chinese cabbage in Korea, were applied to infested soil, club formations by P. brassicae were strongly inhibited at pot (35 $cm^2$) per 0.63 mg. Ethaboxam and cymoxanil, Oomycetes fungicides, completely controlled Chinese cabbage clubroot at 5 mg/pot, but cymoxanil represented sever phytotoxicity. Besides, dichlofluanid and procymidone of botriticides effectively controlled the development of Chinese cabbage clubroot at 2.5 mg/pot. Chlorothalonil, quintozene and trichlamide, broad spectrum fungicides, showed disease-control efficacy of 85%, 100% and 100% at 2.5 mg/pot, respectively. Most of sterol biosynthesis inhibitors displayed the strong antifungal activity against P. brassicae on cabbage seedlings and plant growth -retarding activity. From these results, 7 fungicides were selected and further tested in vivo antifungal activity against P. brassicae in glasshouse. Among them, ethaboxam showed the most antifungal activity against P. brassicae on cabbage seedlings, followed by fenarimol, procymidone, nuarimol and chlorothalonil.

배추 유묘를 Plasmodiophora brassicae 오염토에 이식하고 약제 용액을 폿트(35 $cm^2$)에 관주처리 하는 방법으로 기존 살균제 44종의 배추 뿌리혹병에 대한 방제효과를 실험하였다. 뿌리혹병 방제용 살균제인 fluazinam, flusulfamide 및 cyazofamid는 폿트 당 0.63 mg 처리구에서 모두 90% 이상의 방제가를 나타냈다. 난균류 병해 살균제인 ethaboxam과 cymoxanil은 폿트 당 5 mg 처리 시에 배추 뿌리혹병을 완전히 방제하였다. 그러나 cymoxanil은 고농도 처리에서 배추에 심한 약해를 유발하였다. 잿빛곰팡이병 방제용 살균제인 dichlofluanid와 procymidone도 폿트 당 2.5 mg 처리 시에 배추 뿌리혹병을 효과적으로 방제하였다. 광범위살균제인 chlorothalonil, quintozene과 trichlamide도 2.5 mg 처리 시에 각각 85%, 100%, 100%의 방제가를 나타냈다. 스테롤 생합성 저해제는 대부분 배추 뿌리혹병에 우수한 방제효과를 나타냈으나, 이들은 배추 유묘의 생육을 억제하는 약해를 나타냈다. 이상의 결과로부터 선발된 7종 살균제의 배추 뿌리혹병 방제효과를 비교하여 실험한 결과, 이들 중 ethaboxam은 가장 우수한 방제효과를 나타냈으며 fenarimol, pocymidone, nuarimol, chlorothalonil 순으로 우수한 방제효과를 보였다.

Keywords

References

  1. Arie, T., Y. Kobayashi, G. Okada, Y. Kono and I. Yamaguchi (1998) Control of soilborne clubroot disease of cruciferous plants by epoxydon from Phoma glomerata. Plant Pathol. 47: 743-748 https://doi.org/10.1046/j.1365-3059.1998.00298.x
  2. Braselton, J. (1995) Current status of plasmodiophorids. Crit. Rev. Microbiology 21:263-275 https://doi.org/10.3109/10408419509113543
  3. Burden, R. S., D. T. Cooke and G. A. Carter (1989) Inhibitors of sterol biosynthesis and growth in plants and fungi. Phytochemistry 28:1791-1804 https://doi.org/10.1016/S0031-9422(00)97862-2
  4. Burden, R. S., T. Clark and P. J. Holloway (1987) Effects of sterol biosynthesis-inhibiting fungicides and plant growth regulators on the sterol composition of barley plants. Pestic. Biochem. Physiol. 27 :289-300 https://doi.org/10.1016/0048-3575(87)90058-7
  5. Ikegami, H. (1975) Susceptibility and resistance to clubroot of the cruciferous vegetable species and varieties. Studies on the clubroot of cruciferous plant 1. Res. Bull. Fac. Agric. Gifu Univ. 38: 11-28
  6. Kirk, P. M., P. E. Cannon, J. C. David and J. A. Stalpers (2001) Dictionary of the Fungi, 9th ed. IMI. CAB Internatinal
  7. Komyoji, T., K. Sugimoto, S. Mitani, N. Matsuo and K. Suzuki (1995) Biological properties of a new fungicide, fluazinam. J. Pestic. Sci. 20:129-135
  8. Minami, E. and I. Ando (1994) Analysis of blast disease resistance induced by probenazole in rice. J. Pestic. Sci. 19:79-83
  9. Mitani, S., K. Sugimoto, H. Hayashi, Y. Takii, T. Ohshima and N. Matsuo (2003) Effects of cyazofamid against Plasmodiophora brassicae Woronin on chinese cabbage. Pest Manag. Sci. 59:287-293 https://doi.org/10.1002/ps.627
  10. Nakashita, H., K. Yoshioka, M. Yasuda, T. Nitta, Y. Arai, S. Yoshida and I. Yamaguchi (2002) Probenazole induces systemic acquired resistance in tobacco through salicylic acid accumulation. Physiol. Mol. Plant Pathol. 61:197-203 https://doi.org/10.1006/pmpp.2002.0426
  11. Schaller, H. (2004) New aspects of sterol biosynthesis in growth and development of higher plants. Plant Physiol. Biochem. 42:465-476 https://doi.org/10.1016/j.plaphy.2004.05.012
  12. Shimotori, H., H. Yanagida, Y. Enomoto, K. Igarashi, M. Yoshinari and M. Umemoto (1996) Evaluation of benzenesulfonanilide derivatives for the control of crucifers clubroot. J. Pestic. Sci. 21:31-35
  13. Stanghellini, M. E. and R. M. Miller, (1997) Their identity and potential efficacy in the biological control of zoosporic plant pathogens. Plant Dis. 81:4-12 https://doi.org/10.1094/PDIS.1997.81.1.4
  14. Tanaka S., S. Yoshihara, S. Ito and M. Kameya-Iwaki (1997) The influence of virulence of Plasmodiophora brassicae populations on epidemiology of Chinese cabbage clubroot and efficacy of fungicides. Ann. Phytopathol. Soc. Jpn 63: 183-187 https://doi.org/10.3186/jjphytopath.63.183
  15. Tsuji, G., T. Takeda, I. Furusawa, O. Horino and Y. Kubo (1997) Carpropamid, an anti-rice blast fungicide, inhibits scytalone dehydratase activity and appressorial penetration in Colletotrichum lagenarium. Petic. Biochem. Physiol. 57:211-219 https://doi.org/10.1006/pest.1997.2272
  16. Williams, P. H. (1966) A system for the determination of the races of Plasmodiophora brassicae that infest cabbage and rutabaga. Phytopathology 56:624-626
  17. Yamaguchi, I., S. Sekido and T. Misato (1982) The effect of non-fungicidal anti-blast chemicals on the melanin biosynthesis and infection by Pyricularia oryzae. J. Pestic. Sci. 7:523-529 https://doi.org/10.1584/jpestics.7.523
  18. Yoshigawa, H. (1983) Breeding for clubroot resistance of crucifer crop in Japan. JPN. Agr. Res. Quart. 17: 6-11
  19. Yoshikawa, H. and S. T. Buczacki (1978) Clubroot in Japan: research and problems. Rev. Plant Pathol. 57:253-257
  20. 김두욱, 오정행 (1997) 배추 무사마귀병의 발생상황과 병원균(Plasmodiophora brassicae)의병원성 및 배추 품종의 병저항성. 한국식물병리학회지 13:95-99
  21. 김충회, 조원대, 김홍모 (2000a) 배추무사마귀병균의 토양내 분포. 식물병 연구 6:27-33
  22. 김충회, 조원대, 김홍모 (2000b) 배추무사마귀병균 휴면포자의 발아 및 생존에 미치는 몇가지 환경요인. 한국농약과학회지 4(4):66-71
  23. 조광수, 한영환, 이정태, 허은주, 양태진, 우종규 (2002) 고랭지 지역 배추 무사마귀병원균의 생리형 분화와 저항성 품종 선발. 한국육종학회지 34:168-173
  24. 오정행, 조장환, 김봉구, 채제천, 정길웅, 황철호, 김두욱 (1997) 배추 무사마귀병(Plasmodiophora brassicae)의 발병 유인 및 약제방제. 한국식물병리학회지 13: 244-247