Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Efficacy of Suppression of Phytophthora Blight of Red Pepper Caused by Phytophthora capsici by Treatment with Antagonistic Trichoderma harzianum DYMC in Greenhouse

온실에서 길항미생물 Trichoderma hazianum DYMC 처리에 의한 고추 역병 억제 효과

  • 이용세 (대구대학교 응용생명산업학부) ;
  • 장태현 (상주대학교 식물자원학과) ;
  • 류연주 (대구대학교 응용생명산업학부) ;
  • 박정용 (대구대학교 응용생명산업학부) ;
  • 임태헌 (상주대학교 TIC)
  • Published : 2005.12.31
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Abstract

To control Phytophthora blight of red pepper biologically caused by Phytophthora capsici, we developed Trichoderma harzianum DYMC for commercial product. DYMC was storage at room temperature and was investigated their population every 3 months for 1 year. For investigating the dynamic population of T. harzianum in the pot soils, we applied powder and suspension applications with DYMC, and then investigated for 95 days. The efficacy of powder and suspension applications of DYMC for control of Phytophthora blight of red pepper and plant growth were investigated for 50 days in greenhouse experiment. The population of T. harzianum was decreased at the room temperature for 1 year but there was not statistically significance. After soil treated in the pot with DYMC, the population of Trichoderma spp. was the highest when DYMC powder at 5 g was applied to mix with pot soil, and the population was deceased significantly among treatment means as time goes by ($R^2=0.76$, F=10.5960, P=<.0001). Incidence of Phytophthora blight of, red pepper was significantly reduced among treatment means on 50th day after treated with DYMC ($R^2=0.82$, P=16.4758, P=<.0001). Disease control value was the highest at 62.5% when DYMC powder at 5 g was applied to mix with pot soil. No significant difference (P=0.05) of effects of plant and root growth showed by treated with DYMC on 60th day, except stem. Mixing the application of DYMC powder with soil to control Phytophthora blight of red pepper was greater than suspension application to dilute with water. DYMC could be used as an effective biocontrol agent to control Phythophthora blight of red pepper.

Phytophthora capsici에 의해 발생하는 고추 역병을 생물학적으로 방제하기 위해 상업용 Trichoderma harzianum DYMC를 개발하였다. DYMC는 은박 포장하여 실온에서 1년간 저장하면서 3개월 간격으로 T. harzianum 밀도를 조사하였다. 온실에서 T. harzianum의 활성 밀도를 조사하기 위해 DYMC를 pot 토양과 혼합 한 처리와 현탁액으로 pot에 처리 한 후 95일 동안 조사하였다. 온실에서 고추 역병 방제와 고추 생육에 미치는 효과는 DYMC를 토양과 혼합한 처리와 현탁액으로 pot에 처리한 후50일 동안 조사하였다. 실온에서 T. harzianum 밀도는 1년 동안 경시적으로 감소되었으나 통계적으로 유의성은 없었다. Pot 토양에 Trichoderma spp. 밀도는 DYMC 5 g을 토양과 혼용하여 처리한 구에서 가장 높았으나, 경시적으로 균의 밀도는 통계적으로 유의성 있게 감소되었다($R^2=0.76$, F=10.5960, P=<.0001). 고추역병 발생율은 DYMC 처리 50일에 모든 처리 구에서 유의성있게 감소하였다($R^2=0.82$, F=16.4758, P=<.0001). 병방제가는 DYMC 5 g을 토양과 혼합 처리한 구에서 62.5%로 가장 높았다. 고추생육 효과는 60일 조사에서 줄기 직경을 제외하고는 식물체나 뿌리 생육에서 통계적인 유의차이가 나타나지 않았다 DYMC의 분말을 토양과 혼합하여 시비한 구가 물에 희석하여 현탁액으로 시비한 구 보다 효과가 우수하였다. DYMC 길항 미생물 제제는 고추 역병에 대한 생물학적 방제제로서의 사용 가능성을 보여주었다.

Keywords

References

  1. Hwang, B. K. and Kim, C. H. (1995) Phytophthora blight of pepper and its control in Korea, Plant Dis. 79, 221-227 https://doi.org/10.1094/PD-79-0221
  2. Cook, R. J. (1990) Twenty-five years of progress towards biological control. Pl-4. In D. Hornby (ed.), Biological control of soil-borne plant pathogens, CAB Internaional, Wallingford, UK
  3. Handelsman J. and Stabb, E. V. (1996) Biocontrol of soilborn plant pathogens, Plant Cell. 8, 1855-1869 https://doi.org/10.1105/tpc.8.10.1855
  4. Elad, Y. and Kapat, A. (1999) The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea, Eur. J. Plant Pathol. 105(2), 177-189 https://doi.org/10.1023/A:1008753629207
  5. Howell, C. R., Hanson, L. E., Stipanovic, R. D., and Puckhaber, L. S. (2000) Induction of terpenoid synthesis in cotton roots and control of Rhizoctonia solani by seed treatment with Trichoderma virens, Phytopathology 90(3), 248-252 https://doi.org/10.1094/PHYTO.2000.90.3.248
  6. Yedidia, I., Benhamou, N., and Chet, I. (1999) Induction of defense responses in cucumber plants (Cucumis sativus L.) by the biocontrol agent Triclwdenna harzianum, Appl. Environ. Microbiol. 65(3), 1061-1070
  7. Conway, K. E., Mereddy, R., Kahn, B. A., Wu, Y., and Hallgren, S. W. (2001) Beneficial effects of solid matrix chemo-priming in okra. Plant Dis. 85, 535-537 https://doi.org/10.1094/PDIS.2001.85.5.535
  8. Dandurand, L-M., Mosher, R.D., and Knudsen, G.R. (2000) Combined effects of Brassica napus seed meal and Trichoderma harzianum on two soilborne plant pathogens. Can. J. Microbiol./Rev. Can. Microbiol. 46(11), 1051-1057
  9. Dik, A. and Elad, Y. (1999) Comparison of antagonists of Botrytis cinerea in greenhouse-grown cucumber and tomato under different climatic conditions, Eur. J. Plant Pathol. 105(2), 123-137 https://doi.org/10.1023/A:1008778213278
  10. Dik, A. J., Koning, G., and Koehl, J. (1999) Evaluation of microbial antagonists for biological control of Botrytis cinerea stem infection in cucumber and tomato, Eur. J. Plant Pathol, 105(2), 115-122 https://doi.org/10.1023/A:1008623210258
  11. Ahn, S. J. and Hwang, B. K. (1992) Isolation of antibiotic-producing actinomycetes antagonistic to Phytophthora capsici from pepper-growing soils, Korean J. Mycol. 20, 259-268
  12. Hong, S. S., Park, K. S., Kim, C. H., and Lee, E. J. (1990) Granule formulation of Pseudomonas cepacia antagonistic to Phytophthora capsici and its viability on red-pepper, Korean J. Plant Pathol. 6, 434-439
  13. 황병국, 김은수 (1992) 非病源性 Phytophthora capsici 菌株에 의한 고추疫病의 抑制, 韓國植物病理學會誌 8, 1-7
  14. Jee, H. J., Nam, C. G., and Kim, C. H. (1988) Studies on biological control of Phytophthora blight of red pepper I. Isolation of antagonists and evaluation of antagonistic activity in vitro and in greenhouse, Korean J. Plant Pathol. 4, 305-312
  15. Kim, B. S. and Hwang, B. K. (1992) Isolation of antibiotic-producing bacteria antagonistic to Phytophthora capsici from pepper growing soils and evaluation of their antibiotic activity, Korean J. Plant Pathol. 8, 241-248
  16. Kim, C. H., Kim, K. D., and Jee, H. J. (1991), Enhanced suppression of red-pepper Phyphthora blight by combined applications of antagonist and fungicide, Korean J. Plant Pathol. 7, 221-225
  17. 이인경, 김창진, 김신덕, 유익동 (1990) Streptomyces parvullus 菌株가 生産하는 抗고추역병抗生物質, 韓國應用微生物學會誌. 18, 142-147
  18. Nam, C.G., Jee, H. J., and Kim, C.H. (1988), Studies on biological control of Phytophthora blight of red-pepper II. Enhancement of antagonistic activity by soil amendment with organic materials, Korean J. Plant Pathol. 5, 1-12
  19. Park, H. H. and Kim, H. K. (1989) Biological control of Phytophthora crown and root rot of greenhouse pepper with Thichoderma harzianum and Enterbacter agglomerans by improved method of application, Korean J. Plant Pathol. 5, 1-12
  20. Park, K. S., Hagiwara, H., and Kim, C. H. (1993) Isolation of an antibiotic substance from Pseudomonas cepacia antagonistic to Phytophthora capsici, Korean J. Plant Pathol. 9, 1-6
  21. 박창석 (1989) 미생물에 의한 연작지 염류장해 경감연구, 농시논문집 101-108
  22. Harman, G. E., Chet, I., and Baker, R. (1980) Trichoderma hamatum effects on seedling disease induced in radish and pea by Pythium spp. or Rhizoctonia solani, Phytopathology. 70, 1167-1172 https://doi.org/10.1094/Phyto-70-1167
  23. La, C. T., Nelson, E. B., and Harman, G. E. (1997). Improved biocontrol efficacy of Trichoderma harzianum 1295-22 for foliar phases of turf diseases by use of spray applications. Plant Dis. 81, 1132-1138 https://doi.org/10.1094/PDIS.1997.81.10.1132
  24. Kim, S. I., Shim, J. O., Shin, H. S., Choi, H. J., and Lee, M. W. (1992) Suppressive mechanism of soil-born disease development and its practical application isolation and identification of species of Trichoderma antagonistic to soil diseases and its activities in the rhizosphere. Korean Mychol. 20, 377-346

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